The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca.sup.2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.

The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca.sup.2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.

The present invention relate to three dimensional porous polysaccharide matrices able to induce mineralisation of a tissue in osseous site, as well as in non-osseous site, in the absence of stent cells or growth factors.

Methods and compositions for improving performance of flocculants in an industrial production process. Methods include pH triggered cross-linking reaction between a flocculating agent, such as dextran, and a composition comprising a boronic acid-containing polymer. The pH trigger can be provided by a fluid having a pH of 8 or more. The production process can be a Bayer Process and the fluid is caustic liquor or slurry in the fluid circuit of the Bayer, wherein the reaction time is reduced over conventional methods and the cross-linked dextran composition effectuates improved flocculation of the trihydrate particles.

A chemical blowing agent for foaming a thermoplastic polymer, for example PVC plastisol or a polymer resin in an extrusion process, said chemical blowing agent comprising a functionalized particulate bicarbonate containing at least one additive, preferably excluding an exothermic blowing agent. The additive may be selected from the group consisting of polymers; inorganic salts; oils; fats; resin acids, any derivative thereof, and salts thereof; amino acids; fatty acids; carboxylic or polycarboxylic acids, soaps; waxes; in derivatives thereof (such as esters); salts thereof; or any combinations thereof. The particulate bicarbonate may be functionalized by spray-drying, coating, extrusion or co-grinding with at least one additive. The functionalized particulate bicarbonate may comprise 50 wt % to less than 100 wt % of the bicarbonate component, and 0.02-50 wt % of the additive. A foamable polymer composition comprising such chemical blowing agent. A process for manufacturing a foamed polymer, such as foamed PVC, comprising shaping and heating the foamable polymer composition, and a foamed polymer obtained by such process.

A chemical blowing agent for foaming a thermoplastic polymer, for example PVC plastisol or a polymer resin in an extrusion process, comprising a functionalized particulate bicarbonate containing at least one additive, preferably excluding an exothermic blowing agent. The additive may be selected from the group consisting of polymers; inorganic salts; oils; fats; resin acids, any derivative thereof, and salts thereof; amino acids; fatty acids; carboxylic or polycarboxylic acids, soaps; waxes; derivatives thereof; salts thereof; or any combinations thereof. The particulate bicarbonate may be functionalized by spray-drying, coating, extrusion or co-grinding with at least one additive. The functionalized particulate bicarbonate may comprise 50 wt % to less than 100 wt % of the bicarbonate component, and 0.02-50 wt % of the additive. A foamable polymer composition comprising such chemical blowing agent. A process for manufacturing a foamed polymer, such as foamed PVC, comprising shaping and heating the foamable polymer composition, and a foamed polymer obtained by such process.

Methods and compositions for improving performance of flocculants in an industrial production process. Methods include pH triggered cross-linking reaction between a flocculating agent, such as dextran, and a composition comprising a boronic acid-containing polymer. The pH trigger can be provided by a fluid having a pH of 8 or more. The production process can be a Bayer Process and the fluid is caustic liquor or slurry in the fluid circuit of the Bayer, wherein the reaction time is reduced over conventional methods and the cross-linked dextran composition effectuates improved flocculation of the trihydrate particles.

A method of coating a polymer surface, the method comprising: providing a polymer having a surface, optionally, treating at least a portion of the polymer surface with an oxidising agent, treating at least a portion of the polymer surface with a composition comprising a polysaccharide, oligosaccharide, polyol or mixture thereof, and incubating the treated polymer with the composition for a predetermined time. Also disclosed are polymers comprising such a coating, vessels comprising such coated polymers and medicals devices comprising such polymers.

Methods and compositions for improving performance of flocculants in an industrial production process. Methods include pH triggered cross-linking reaction between a flocculating agent, such as dextran, and a composition comprising a boronic acid-containing polymer. The pH trigger can be provided by a fluid having a pH of 8 or more. The production process can be a Bayer Process and the fluid is caustic liquor or slurry in the fluid circuit of the Bayer, wherein the reaction time is reduced over conventional methods and the cross-linked dextran composition effectuates improved flocculation of the trihydrate particles.

A chemical blowing agent for foaming a thermoplastic polymer, for example PVC plastisol or a polymer resin in an extrusion process, comprising a functionalized particulate bicarbonate containing at least one additive, preferably excluding an exothermic blowing agent. The additive may be selected from the group consisting of polymers; inorganic salts; oils; fats; resin acids, any derivative thereof, and salts thereof; amino acids; fatty acids; carboxylic or polycarboxylic acids, soaps; waxes; derivatives thereof; salts thereof; or any combinations thereof. The particulate bicarbonate may be functionalized by spray-drying, coating, extrusion or co-grinding with at least one additive. The functionalized particulate bicarbonate may comprise 50 wt % to less than 100 wt % of the bicarbonate component, and 0.02-50 wt % of the additive. A foamable polymer composition comprising such chemical blowing agent. A process for manufacturing a foamed polymer, such as foamed PVC, comprising shaping and heating the foamable polymer composition, and a foamed polymer obtained by such process.