Provided is a diagnostic kit for determining infection with Methicillin-Resistant Staphylococcus aureus (MRSA) in a specimen, and a method for determining the infection with MRSA using the diagnostic kit is performed by visually observing a color change after LAMP reaction, and the color change is caused by a change in a magnesium concentration and confirmed using a specific dye compound which sensitively reacts with magnesium ions. The amplification of the MRSA DNA is performed using the loop-mediated isothermal amplification (LAMP), so that the diagnostic kit has advantages of being conveniently used anytime and anywhere and quickly diagnosing.

Provided is a diagnostic kit for determining infection with Methicillin-Resistant Staphylococcus aureus (MRSA) in a specimen, and a method for determining the infection with MRSA using the diagnostic kit is performed by visually observing a color change after LAMP reaction, and the color change is caused by a change in a magnesium concentration and confirmed using a specific dye compound which sensitively reacts with magnesium ions. The amplification of the MRSA DNA is performed using the loop-mediated isothermal amplification (LAMP), so that the diagnostic kit has advantages of being conveniently used anytime and anywhere and quickly diagnosing.

A dye mixture comprising at least one dye of formula (formula) and at least one dye of formula (formula), wherein R.sub.1 is hydrogen or C.sub.1-C.sub.4alkyl, R.sub.2 is hydrogen or C.sub.1-C.sub.4alkyl, C.sub.1-C.sub.4alkoxy or sulfo, Q.sub.1 is a fibre-reactive radical of formula —(CH.sub.2).sub.2-3—SO.sub.2—Y.sub.2 (3a) or —(CH.sub.2).sub.2-3—O—(CH.sub.2).sub.2-3—SO.sub.2—Y.sub.3 (3b), Q.sub.2 has the meaning of Q.sub.1, or is hydrogen or C.sub.1-C.sub.4alkyl, X.sub.1 and X.sub.2 are each independently of the other halogen, 3-carboxypyridin-1-yl or 3-carbamoylpyridin-1-yl, Y.sub.1, Y.sub.2 and Y.sub.3 are each independently of the others vinyl or a radical —CH.sub.2—CH.sub.2—U and U is a group removable under alkaline conditions, Z.sub.1 and Z.sub.2 are each independently of the other —S0.sub.2-Y, wherein Y is vinyl or a radical —CH.sub.2—CH.sub.2—U and U is a group removable under alkaline conditions, M is hydrogen, an alkali metal or one equivalent of an alkaline earth metal, k and n are each independently of the other the number 0, 1 or 2, I and q are each independently of the other the number 0 or 1, m is the number 0 or 1, and wherein the diazo component of the dye of formula (1) and the diazo component of the dye of formula (2) each independently of the other are a benzene radical or a naphthyl radical, are suitable especially for the dyeing or printing of cellulosic fibre materials, exhibit a good build-up, a good fixation rate and good washing off properties of the unfixed dyes, and good all-round fastness properties.

##STR00001##

A dye mixture comprising at least one dye of formula (formula) and at least one dye of formula (formula), wherein R.sub.1 is hydrogen or C.sub.1-C.sub.4alkyl, R.sub.2 is hydrogen or C.sub.1-C.sub.4alkyl, C.sub.1-C.sub.4alkoxy or sulfo, Q.sub.1 is a fibre-reactive radical of formula —(CH.sub.2).sub.2-3—SO.sub.2—Y.sub.2 (3a) or —(CH.sub.2).sub.2-3—O—(CH.sub.2).sub.2-3—SO.sub.2—Y.sub.3 (3b), Q.sub.2 has the meaning of Q.sub.1, or is hydrogen or C.sub.1-C.sub.4alkyl, X.sub.1 and X.sub.2 are each independently of the other halogen, 3-carboxypyridin-1-yl or 3-carbamoylpyridin-1-yl, Y.sub.1, Y.sub.2 and Y.sub.3 are each independently of the others vinyl or a radical —CH.sub.2—CH.sub.2—U and U is a group removable under alkaline conditions, Z.sub.1 and Z.sub.2 are each independently of the other —S0.sub.2-Y, wherein Y is vinyl or a radical —CH.sub.2—CH.sub.2—U and U is a group removable under alkaline conditions, M is hydrogen, an alkali metal or one equivalent of an alkaline earth metal, k and n are each independently of the other the number 0, 1 or 2, I and q are each independently of the other the number 0 or 1, m is the number 0 or 1, and wherein the diazo component of the dye of formula (1) and the diazo component of the dye of formula (2) each independently of the other are a benzene radical or a naphthyl radical, are suitable especially for the dyeing or printing of cellulosic fibre materials, exhibit a good build-up, a good fixation rate and good washing off properties of the unfixed dyes, and good all-round fastness properties.

##STR00001##

Disclosed is a compound of formula (I):

##STR00001##

wherein L, R.sup.1-R.sup.5, A, B, M, and n are as defined in the specification, as well as a method of preparing the compound. Also disclosed are a method of blood-pool imaging in a mammal and a method of imaging a lymph node in a mammal, comprising use of the compound.

Disclosed is a compound of formula (I):

##STR00001##

wherein L, R.sup.1-R.sup.5, A, B, M, and n are as defined in the specification, as well as a method of preparing the compound. Also disclosed are a method of blood-pool imaging in a mammal and a method of imaging a lymph node in a mammal, comprising use of the compound.

The present invention provides an ink jet recording ink including a dye which has a weight average molecular weight of 850 or less and is represented by Formula (1) below, a water-soluble organic solvent which has an SP value of 9.4 or more and less than 9.75 and is represented by Formula (2) or (3) below, and water, in which the content of a surfactant is less than 0.1% by mass. R.sub.1, R.sub.2: H, a halogen atom, OH, COOH, C1-C6 alkyl group, C1-C6 alkoxy group, NO.sub.2, an azophenyl group, -L1-Ra and the like, L.sub.1: O, CH.sub.2, C.sub.6H.sub.4, N═N, SO.sub.2 or a combination thereof, Ra: a phenyl group, a naphthyl group, or the like, R.sub.3: H, an azophenyl group, an azonaphthalene group, -L.sub.2-Rb or the like, L.sub.2: C(═O), C.sub.6H.sub.4, N═N, NH, SO.sub.2 or a combination thereof, Rb: a phenyl group, a naphthyl group, a pyrimidyl group, or the like; M.sup.+: H.sup.+, Na.sup.+, NH.sub.4.sup.+, an alkylammonium ion or the like, R: C2-C6 unsubstituted alkyl group, and n is 1 to 3. ##STR00001##

The present invention provides an ink jet recording ink including a dye which has a weight average molecular weight of 850 or less and is represented by Formula (1) below, a water-soluble organic solvent which has an SP value of 9.4 or more and less than 9.75 and is represented by Formula (2) or (3) below, and water, in which the content of a surfactant is less than 0.1% by mass. R.sub.1, R.sub.2: H, a halogen atom, OH, COOH, C1-C6 alkyl group, C1-C6 alkoxy group, NO.sub.2, an azophenyl group, -L1-Ra and the like, L.sub.1: O, CH.sub.2, C.sub.6H.sub.4, N═N, SO.sub.2 or a combination thereof, Ra: a phenyl group, a naphthyl group, or the like, R.sub.3: H, an azophenyl group, an azonaphthalene group, -L.sub.2-Rb or the like, L.sub.2: C(═O), C.sub.6H.sub.4, N═N, NH, SO.sub.2 or a combination thereof, Rb: a phenyl group, a naphthyl group, a pyrimidyl group, or the like; M.sup.+: H.sup.+, Na.sup.+, NH.sub.4.sup.+, an alkylammonium ion or the like, R: C2-C6 unsubstituted alkyl group, and n is 1 to 3. ##STR00001##

Provided is a diagnostic kit for determining infection with Methicillin-Resistant Staphylococcus aureus (MRSA) in a specimen, and a method for determining the infection with MRSA using the diagnostic kit is performed by visually observing a color change after LAMP reaction, and the color change is caused by a change in a magnesium concentration and confirmed using a specific dye compound which sensitively reacts with magnesium ions. The amplification of the MRSA DNA is performed using the loop-mediated isothermal amplification (LAMP), so that the diagnostic kit has advantages of being conveniently used anytime and anywhere and quickly diagnosing.

Provided is a diagnostic kit for determining infection with Methicillin-Resistant Staphylococcus aureus (MRSA) in a specimen, and a method for determining the infection with MRSA using the diagnostic kit is performed by visually observing a color change after LAMP reaction, and the color change is caused by a change in a magnesium concentration and confirmed using a specific dye compound which sensitively reacts with magnesium ions. The amplification of the MRSA DNA is performed using the loop-mediated isothermal amplification (LAMP), so that the diagnostic kit has advantages of being conveniently used anytime and anywhere and quickly diagnosing.