The present application relates to photoacoustic agents, to kits comprising such photoacoustic agents and to uses thereof such as in methods for detecting a presence of a target in a subject. The photoacoustic agents comprise a photoacoustically active moiety (for example, a near infrared dye) that is coupled to a first bioorthogonal reactive group and optionally a targeting entity for a target that is coupled to a second bioorthogonal reactive group.

An ink jet recording method including the steps of a first recording step for applying a first ink to a recording medium and a second recording step for applying a second ink to the recording medium so as to overlap at least a region provided with the first ink, wherein the first ink is an aqueous ink containing silver particles, the second ink is an aqueous ink containing a dye, a time difference between applications of the first ink and the second ink to the recording medium is 1 sec or more to 7,200 sec or less, and the dye is a predetermined dye.

An ink jet recording method including the steps of a first recording step for applying a first ink to a recording medium and a second recording step for applying a second ink to the recording medium so as to overlap at least a region provided with the first ink, wherein the first ink is an aqueous ink containing silver particles, the second ink is an aqueous ink containing a dye, a time difference between applications of the first ink and the second ink to the recording medium is 1 sec or more to 7,200 sec or less, and the dye is a predetermined dye.

An ink jet recording method including the steps of a first recording step for applying a first ink to a recording medium and a second recording step for applying a second ink to the recording medium so as to overlap at least a region provided with the first ink, wherein the first ink is an aqueous ink containing silver particles, the second ink is an aqueous ink containing a dye, a time difference between applications of the first ink and the second ink to the recording medium is 1 sec or more to 7,200 sec or less, and the dye is a predetermined dye.

An ink jet recording method including the steps of a first recording step for applying a first ink to a recording medium and a second recording step for applying a second ink to the recording medium so as to overlap at least a region provided with the first ink, wherein the first ink is an aqueous ink containing silver particles, the second ink is an aqueous ink containing a dye, a time difference between applications of the first ink and the second ink to the recording medium is 1 sec or more to 7,200 sec or less, and the dye is a predetermined dye.

The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: ##STR00001##
wherein the variables are described throughout the application.