A bio-ink comprising: (a) at least one polysaccharide; (b) at least one structural protein; and (c) fibrinogen, wherein the polysaccharide is selected from a group consisting of Sodium alginate Chitosan, Hyaluronic acid, Gellan gum, Dextran, Agarose, Poly(ethylene glycol), Pluronic and Carrageenan and the structural protein is selected from a group consisting of Gelatin, Collagen Vitronectin, Laminins and Fibronectin.

A bio-ink comprising: (a) at least one polysaccharide; (b) at least one structural protein; and (c) fibrinogen, wherein the polysaccharide is selected from a group consisting of Sodium alginate Chitosan, Hyaluronic acid, Gellan gum, Dextran, Agarose, Poly(ethylene glycol), Pluronic and Carrageenan and the structural protein is selected from a group consisting of Gelatin, Collagen Vitronectin, Laminins and Fibronectin.

The invention relates to compositions comprising a keratin pigment material. The keratin pigment material comprises dyed keratin particles. Compositions comprising keratin pigment material such as inks, paints and cosmetic formulations have advantageous properties compared to similar compositions in which conventional pigment materials are used as colourants.

The invention relates to compositions comprising a keratin pigment material. The keratin pigment material comprises dyed keratin particles. Compositions comprising keratin pigment material such as inks, paints and cosmetic formulations have advantageous properties compared to similar compositions in which conventional pigment materials are used as colourants.

The present disclosure is generally directed to a bio-ink system that can be used to print cellularized scaffolds for biomedical applications. In certain configurations, the ink has rheological properties suitable for extrusion-based printing techniques and adipose stem cells infused in the ink remain viable seven days after printing. The ink may comprise a mixture of alginate hydrogels, which provide the structural integrity of the printed scaffolds, and gelatin hydrogels, which support the cell proliferation. Borophosphate glass particles are added to the hydrogel mixture where they release Ca-ions that control the viscoelastic properties of the hydrogel before and after printing. Borophosphate glasses described herein promote significantly better ASC viability than previously employed glasses.

The present disclosure is generally directed to a bio-ink system that can be used to print cellularized scaffolds for biomedical applications. In certain configurations, the ink has rheological properties suitable for extrusion-based printing techniques and adipose stem cells infused in the ink remain viable seven days after printing. The ink may comprise a mixture of alginate hydrogels, which provide the structural integrity of the printed scaffolds, and gelatin hydrogels, which support the cell proliferation. Borophosphate glass particles are added to the hydrogel mixture where they release Ca-ions that control the viscoelastic properties of the hydrogel before and after printing. Borophosphate glasses described herein promote significantly better ASC viability than previously employed glasses.

A photocrosslinkable bioink composition for three-dimensional printing is provided. The photocrosslinkable bioink composition includes: an aqueous solution of a recombinant spider silk protein comprising the NT2RepCT-MaSp2 sequence; a polymer comprising pendant groups reactive under visible light-initiated radical polymerization; and a photoinitiator system configured to generate free radicals upon exposure to visible light. The polymer is selected from the group consisting of methacrylated gelatin, methacrylated hyaluronic acid, methacrylated chitosan, polyethylene glycol diacrylate, and combinations thereof. The photoinitiator system is selected from the group consisting of tris(bipyridine)ruthenium(II) chloride with ammonium persulfate, eosin Y with triethanolamine, riboflavin with ammonium persulfate, and lithium phenyl-2,4,6-trimethylbenzoylphosphinate. The photocrosslinkable bioink composition is curable under visible light to form a biocompatible hydrogel structure suitable for cell culture or implantation.

A photocrosslinkable bioink composition for three-dimensional printing is provided. The photocrosslinkable bioink composition includes: an aqueous solution of a recombinant spider silk protein comprising the NT2RepCT-MaSp2 sequence; a polymer comprising pendant groups reactive under visible light-initiated radical polymerization; and a photoinitiator system configured to generate free radicals upon exposure to visible light. The polymer is selected from the group consisting of methacrylated gelatin, methacrylated hyaluronic acid, methacrylated chitosan, polyethylene glycol diacrylate, and combinations thereof. The photoinitiator system is selected from the group consisting of tris(bipyridine)ruthenium(II) chloride with ammonium persulfate, eosin Y with triethanolamine, riboflavin with ammonium persulfate, and lithium phenyl-2,4,6-trimethylbenzoylphosphinate. The photocrosslinkable bioink composition is curable under visible light to form a biocompatible hydrogel structure suitable for cell culture or implantation.