The subject invention relates to methods for post-primary oil recovery that utilize multiple mechanisms of action, wherein biochemical-producing microbes are injected into an oil well in a certain predetermined order, based on, for example, the metabolites and by-products they are capable of producing. Preferably, gas-, acid- and/or solvent-producing microbe such as a Clostridium bacterium, is injected into the well first, followed by a biosurfactant and/or biopolymer-producing microbe, such as Bacillus subtilis, Bacillus licheniformis and/or a combination thereof.

The subject invention relates to methods for post-primary oil recovery that utilize multiple mechanisms of action, wherein biochemical-producing microbes are injected into an oil well in a certain predetermined order, based on, for example, the metabolites and by-products they are capable of producing. Preferably, gas-, acid- and/or solvent-producing microbe such as a Clostridium bacterium, is injected into the well first, followed by a biosurfactant and/or biopolymer-producing microbe, such as Bacillus subtilis, Bacillus licheniformis and/or a combination thereof.

Compositions and methods are provided for use in controlling souring and corrosion causing prokaryotes, such as SRP, by treating oil and gas field environments or treatment fluids with a newly identified bacterial strain ATCC Accession No. PTA-124262 as a self-propagating whole cell that produces an anti-SRP bacteriocin in situ. In another aspect, the methods use one or more toxic peptides or proteins isolated therefrom in methods to control unwanted prokaryotic growth in these environments.

Compositions and methods are provided for use in controlling souring and corrosion causing prokaryotes, such as SRP, by treating oil and gas field environments or treatment fluids with a newly identified bacterial strain ATCC Accession No. PTA-124262 as a self-propagating whole cell that produces an anti-SRP bacteriocin in situ. In another aspect, the methods use one or more toxic peptides or proteins isolated therefrom in methods to control unwanted prokaryotic growth in these environments.

A biochemical viscosity reducer for heavy oil and a preparation method thereof. The viscosity reducer includes: Brevibacillus borstelensis-fermented mixed lipopeptide solution: 30 to 60 parts; compound biological enzyme: 15 to 30 parts; plant-based nonionic surfactant: 10 to 20 parts; antibacterial agent: 1 to 5 parts; stabilizer: 1 to 5 parts; and alcohol solvent: 10 to 20 parts; where, the above components are measured by mass. The preparation method includes: step 1: adding 30 to 60 parts of a Brevibacillus borstelensis-fermented mixed lipopeptide solution, 15 to 30 parts of a compound biological enzyme, 10 to 20 parts of a plant-based nonionic surfactant, and 1 to 5 parts of a stabilizer to a reactor; and step 2: adding 1 to 5 parts of an antibacterial agent and an alcohol solvent to the reactor, and stirring a resulting mixture for 60 min to 120 min.

A biochemical viscosity reducer for heavy oil and a preparation method thereof. The viscosity reducer includes: Brevibacillus borstelensis-fermented mixed lipopeptide solution: 30 to 60 parts; compound biological enzyme: 15 to 30 parts; plant-based nonionic surfactant: 10 to 20 parts; antibacterial agent: 1 to 5 parts; stabilizer: 1 to 5 parts; and alcohol solvent: 10 to 20 parts; where, the above components are measured by mass. The preparation method includes: step 1: adding 30 to 60 parts of a Brevibacillus borstelensis-fermented mixed lipopeptide solution, 15 to 30 parts of a compound biological enzyme, 10 to 20 parts of a plant-based nonionic surfactant, and 1 to 5 parts of a stabilizer to a reactor; and step 2: adding 1 to 5 parts of an antibacterial agent and an alcohol solvent to the reactor, and stirring a resulting mixture for 60 min to 120 min.

The subject invention provides multi-functional biochemical compositions, as well as their use in enhancing oil recovery from an oil-bearing subterranean formation. Advantageously, the compositions and methods of the subject invention are operationally-friendly, cost-effective, and environmentally-friendly. More specifically, in preferred embodiments, the subject invention provides a multi-functional composition for enhanced oil recovery (EOR) comprising one or more surfactants, one or more chelating agents, and one or more solvents.

The present invention relates to a biological and chemical composite blockage removing agent and preparation process and use thereof, and mainly solves the problems that the existing blockage removing agents have poor blockage removal effect on organic blockages, cause damage to formations and oil-wells, produce serious environmental pollution, and corrode the equipment, pipelines, and the like. The problems can be well solved by using a technical solution of a biological and chemical composite blockage removing agent, which contains the following components in parts by mass: A. 10-50 parts of a biosurfactant; B. 5-20 parts of a chemical surfactant; wherein the chemical surfactant is an anionic-nonionic surfactant, and said technical solution can be used in the industrial production for removing the blockage in the oilfield, and reducing the injection pressure and increasing the injection.

Compositions and methods are provided for use in controlling souring and corrosion causing prokaryotes, such as SRP, by treating oil and gas field environments or treatment fluids with a newly identified bacterial strain ATCC Accession No. PTA-124262 as a self-propagating whole cell that produces an anti-SRP bacteriocin in situ. In another aspect, the methods use one or more toxic peptides or proteins isolated therefrom in methods to control unwanted prokaryotic growth in these environments.

Compositions and methods are provided for use in controlling souring and corrosion causing prokaryotes, such as SRP, by treating oil and gas field environments or treatment fluids with a newly identified bacterial strain ATCC Accession No. PTA-124262 as a self-propagating whole cell that produces an anti-SRP bacteriocin in situ. In another aspect, the methods use one or more toxic peptides or proteins isolated therefrom in methods to control unwanted prokaryotic growth in these environments.