A method for preparing high-content conjugated linoleic acid (CLA) through Purification of vegetable oil includes alcoholysis, purification and isomerization of vegetable oil. Alcoholysis is for preparing corresponding methyl ester or ethyl ester with glyceride; purification of methyl ester or ethyl ester is for obtaining methyl linoleate or ethyl linoleate of content over 85% through silver-based silica gel column chromatography; high-content CLA is obtained after alkali-catalyzed conjugation of methyl linoleate or ethyl linoleate, and CLA products are prepared as needed. This invention changes the status quo of preparing high-content CLA with safflower oil alone, expands sources of CLA, and develops an efficient technology for separation and purification of linoleic acid. The CLA obtained is of high purity and meets applications in pharmaceutical, health care products and other industries.

A method of producing a composition comprising a high concentration of very long chain polyunsaturated fatty acids (VLCPUFAs) from natural oils such as fish oil, squid oil, algal oil and krill oil. In addition, a composition comprising a high concentration of very long chain polyunsaturated fatty acids isolated from such natural sources; as well as to a process for isolating separate fractions of very long chain polyunsaturated fatty acids having identical chain lengths but different degrees of unsaturation from such highly concentrated compositions is disclosed.

Agricultural residues (biomasses) have come-up as potential valuable renewable resources for transformation into many bio-products. To achieve this goal, the isolation of major biopolymers in their purest form through an economical process is demanded for synthesis of the target fine chemicals without impurities. However, the difficulty of fractionating the biomass into cellulose, hemicellulose and lignin by a simple method has greatly limited their isolation from agricultural residues. Also, the lipids and waxes present on the surface of the agricultural residues protect it from external chemical and microbial agents. To overcome this issue, the lipids and waxes have been isolated from the biomass through two step organic solvents extraction. Thereafter lignin, hemicellulose and cellulose are isolated successively from the de-waxed biomass.

Triglyceride oils having one or more populations of asymmetric triglyceride molecules are provided. Asymmetric triglyceride molecule populations are triglyceride molecules that consist of a C8:0 fatty acid or a C10:0 fatty acid at the sn-1 position and the sn-2 position, and C16:0 or C18:0 at the sn-3 position. Another population of asymmetric triglyceride molecules are triglyceride molecules that consist of a C16:0 fatty acid or a C18:0 fatty acid at the sn-1 position and the sn-2 position, and C8:0 or C10:0 fatty acid at the sn-3 position. Methods of producing triglyceride oils and using the same are provided using sucrose invertase and hydrogenation of the triglyceride oil. Triglyceride molecules are produced by using recombinant DNA techniques to produce oleaginous recombinant cells.

Agricultural residues (biomasses) have come-up as potential valuable renewable resources for transformation into many bio-products. To achieve this goal, the isolation of major biopolymers in their purest form through an economical process is demanded for synthesis of the target fine chemicals without impurities. However, the difficulty of fractionating the biomass into cellulose, hemicellulose and lignin by a simple method has greatly limited their isolation from agricultural residues. Also, the lipids and waxes present on the surface of the agricultural residues protect it from external chemical and microbial agents. To overcome this issue, the lipids and waxes have been isolated from the biomass through two step organic solvents extraction. Thereafter lignin, hemicellulose and cellulose are isolated successively from the de-waxed biomass.

A method for preparing high-content conjugated linoleic acid (CLA) through purification of vegetable oil includes alcoholysis, purification and isomerization of vegetable oil. Alcoholysis is for preparing corresponding methyl ester or ethyl ester with glyceride; purification of methyl ester or ethyl ester is for obtaining methyl linoleate or ethyl linoleate of content over 85% through silver-based silica gel column chromatography; high-content CLA is obtained after alkali-catalyzed conjugation of methyl linoleate or ethyl linoleate, and CLA products are prepared as needed. This invention changes the status quo of preparing high-content CLA with safflower oil alone, expands sources of CLA, and develops an efficient technology for separation and purification of linoleic acid. The CLA obtained is of high purity and meets applications in pharmaceutical, health care products and other industries.

A method of producing a composition comprising a high concentration of very long chain polyunsaturated fatty acids (VLCPUFAs) from natural oils such as fish oil, squid oil, algal oil and krill oil. In addition, a composition comprising a high concentration of very long chain polyunsaturated fatty acids isolated from such natural sources; as well as to a process for isolating separate fractions of very long chain polyunsaturated fatty acids having identical chain lengths but different degrees of unsaturation from such highly concentrated compositions is disclosed.

[PROBLEM TO BE SOLVED] To provide a method for producing ether phospholipids of high purity by an easy process. [SOLUTION] This production method comprises: (A) a step of extracting total lipids from organisms with non-polar organic solvent and branched alcohol mixture; and (B) a step of reacting the total lipids obtained by the step (A) with phospholipase A1 to decompose mixed diacyl-glycerophospholipids. This makes it possible to produce plasmalogen-type glycerophospholipids or ether phospholipids of high purity by an easy process.