The present disclosure provides lipolytic enzyme variants having two or more modifications as compared to a parent lipolytic enzyme. Specifically, the present disclosure provides one or more lipolytic enzyme variants that has at least one improved performance when compared to one or more reference lipolytic enzymes. In addition, the present disclosure provides compositions comprising a lipolytic enzyme variant of the disclosure. The present disclosure also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the disclosure.

The disclosure relates to products including a polysaccharide derivative wherein the polysaccharide derivative includes a polysaccharide substituted with at least one of: a) at least one sulfate group; b) at least one sulfonate group; c) at least one thiosulfate group; or d) a combination thereof; wherein the polysaccharide is poly alpha-1,3-glucan, poly alpha-1,6-glucan, poly alpha-1,3-1,6-glucan, or a mixture thereof; and the polysaccharide derivative has a degree of substitution of about 0.001 to about 3. The products can be useful as anti-deposition and/or anti-graying agents in laundry detergents, and in home care applications.

A liquid bioactive composition is provided that includes an enzyme with enzymatic activity toward a component of a biological stain. Also provided is a process for facilitating the removal of a biological stain is provided wherein the enzyme in the liquid bioactive coating material is capable of enzymatically degrading of one or more components of the biological stain to facilitate biological stain removal from a substrate.

Disclosed herein are compositions including (A) at least one polymer including (a) a core obtained by reacting a diamine H.sub.2N—Z—NH.sub.2 with a compound having a total number TN of carboxyl groups per molecule in the range of from 3 to 10, where Z is selected from C.sub.3-C.sub.12-cycloalkylene that may be non-substituted or substituted with one or more O—C.sub.1-C.sub.4-alkyl groups and where C.sub.3-C.sub.12-cycloalkylene may bear one to three methyl groups, and where carboxyl groups are selected from COOH and COOX.sup.1 with X.sup.1 being selected from C.sub.1-C.sub.4-alkyl, or two carboxyl groups together form an anhydride group, (b) polyalkylene oxide chains, and (B) at least one hydrolase.

Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

A fluid washing composition with four separate components and laundry washing kit thereof is disclosed. The four components are housed in respective independent tanks of a laundry washing machine equipped with a program for selecting, dosing and differentiated delivering. Component 1 is a detergent formulation with a pH of 4.0-12.0, with high detergent effect in washing programs at temperatures of 40°-60° C., having a specific action on cotton fibers, particularly, whites. Component 2 is a detergent formulation with a pH of 8.0-12.0, with high detergent effect in washing programs at temperatures of 15°-40° C., having a specific action on synthetic, colored and animal-origin fibers. Component 3 is an enzyme-based stain-removing formulation including protease and lipase and possibly amylase with a pH of 5.0-6.0, stabilized with sorbitol and calcium chloride. Component 4 is an after-wash conditioning formulation, with a pH of 2.0-4.0.

The invention provides an enzymatic water-in-oil emulsion, which is suitable for making multi-enzyme blends, and for producing enzymatic detergents.

A series of natural soaps, both liquid and solid, and methods of making thereof, which have a synergistic effect when formulated with anti-redeposition ingredients in laundry applications both in synthetic and natural textiles and fabrics. The fatty acids and/or natural oils based series of natural soaps include both sodium and potassium soap products that interact synergistically with, preferably, known, natural anti-redeposition agents and alkaline builders creating highly effective, natural solid (bar or powdered) and liquid laundry cleaning products. Solid forms soaps are dried to an acceptable amount of moisture content for milling. Further blending is then performed with various natural anti-redeposition agents or natural products which exhibit said characteristics and/or alkaline builders. The solid forms may be formed into single dose tablets. Effervescent additives may further be included.

Disclosed herein are embodiments of an enzyme composition for use in cleaning components of biomass-based processing. In some embodiments, the enzyme composition comprises a surfactant or is used in combination with a surfactant-containing composition. The enzyme composition can be administered to a process stream or directly to a process screen used in biomass-based processing to facilitate removing starch build-up on the process screen.

The present invention relates to new polypeptides, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides. The present invention also relates to detergent composition comprising polypeptides, a laundering method and the use of polypeptides.