Disclosed is variants of a cellulase having improved stability in the presence of a protease, and the use of such variants in laundry.

The disclosure is directed to cleaning compositions, methods of making the cleaning compositions, and methods of using the cleaning compositions. The cleaning compositions comprise an enzyme composition, a nonionic surfactant, and a quaternary amine. Preferably, the enzyme compositions included in the cleaning compositions comprise a cellulase, an AA9 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, a swollenin, or a combination or mixture thereof. The compositions are useful for degradation of bacterial cellulose.

A fluid washing composition with four separate components and laundry washing kit thereof is disclosed. The four components are housed in respective independent tanks of a laundry washing machine equipped with a program for selecting, dosing and differentiated delivering. Component 1 is a detergent formulation with a pH of 4.0-12.0, with high detergent effect in washing programs at temperatures of 40°-60° C., having a specific action on cotton fibers, particularly, whites. Component 2 is a detergent formulation with a pH of 8.0-12.0, with high detergent effect in washing programs at temperatures of 15°-40° C., having a specific action on synthetic, colored and animal-origin fibers. Component 3 is an enzyme-based stain-removing formulation including protease and lipase and possibly amylase with a pH of 5.0-6.0, stabilized with sorbitol and calcium chloride. Component 4 is an after-wash conditioning formulation, with a pH of 2.0-4.0.

The invention provides a liquid boron-free enzyme composition, comprising aliphatic 1,2-diols, which exhibit excellent physical and microbial stability.

Described herein is a liquid enzyme preparation containing component (a): at least one salt according to general formula (I)
(R.sup.2).sub.3N.sup.+—(CH.sub.2).sub.nC(R.sup.3)(R.sup.4)—(O—X).sub.m—O—C(O)—R.sup.1A.sup.−  (I) wherein n is selected from 1 to 12, m is selected from zero to 50, R.sup.1 is selected from the group consisting of methyl, ethyl, —CH(OH)—CH(OH)—COOH, CH(OH)—CH.sub.3, (E)-CH═CHCOOH, (Z)—CH═CHCOOH, —C.sub.6H.sub.5, para-HO—C.sub.6H.sub.4—, o,p-dihydroxyphenyl, and 3,4,5-triydroxyphenyl, R.sup.2 are same or different and selected from C.sub.1-C.sub.10-alkyl, phenyl, R.sup.3 and R.sup.4 are same or different and selected from hydrogen and C.sub.1-C.sub.4-alkyl, X is C.sub.2-C.sub.4-alkylen, and A.sup.− is an inorganic or organic counteranion,
component (b): at least one enzyme selected from hydrolases (EC 3),
and
optionally component (c): at least one compound selected from enzyme stabilizers different from component (a), preservatives, and surfactants.

The disclosure is directed to cleaning compositions, methods of making the cleaning compositions, and methods of using the cleaning compositions. The cleaning compositions comprise an enzyme composition, a nonionic surfactant, and a quaternary amine. Preferably, the enzyme compositions included in the cleaning compositions comprise a cellulase, an AA9 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, a swollenin, or a combination or mixture thereof. The compositions are useful for degradation of bacterial cellulose.

Disclosed herein is one or more subtilisin variant, nucleic acid encoding same, and compositions and methods related to the production and use thereof, including one or more Bacillus gibsonii-clade subtilisin variant that has improved stability and/or soil removal compared to one or more reference subtilisin.

A mannanase at least 75% identical to SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, a polynucleotide encoding the mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.

The present invention relates to compositions, methods and use of a mixture of enzymes having DNase activity and an enzyme having hexosaminidase activity.

The present invention relates to polypeptides having hexosaminidase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.