A method for optimizing a process (PROC) to produce a biochemical product (P) defined by a quality attribute, the process being controlled by an actuation parameter (C) and being monitored to get a measured value (T). The method includes training a predictive model (PRED) on a training database; and deploying the trained predictive model (PRED) to provide a correction actuation parameter (dC) when a predicted quality attribute (pQA) is out of a targeted quality attribute interval (QAmin, QAmax). The method also includes a step of designing a physical model of the process (PROC) able to provide a simulated quality attribute, the training database comprising simulated quality attributes computed from the physical model and experimental quality attributes computed from biochemical products (P) previously produced.

A stirring device of a plug-flow fermentation device includes a shaft rotatable about an axis of rotation which defines an axial direction. The stirring device further includes a boundary stirring element that defines the axial extent of a stirring volume covered by the stirring device. A nearest neighbor of the boundary stirring element in the axial direction has an axial maximum width which is smaller than an axial maximum width of the boundary stirring element and which is larger than an axial maximum width of a next-nearest neighbor of the boundary stirring element.

Bioreactor systems can include a first frame and a second frame, a well plate, a motor plate, a motor, and a controller. The first frame may define a well plate inset and standoff insets for a first set of metal standoffs. The second frame may define a plurality of mounts and a plurality of insets for a second set of metal standoffs. A gear may be positioned in each of the plurality of mounts. A paddle may be coupled to each of the gears. The well plate can be positioned within the well plate inset. The motor plate can be supported by and connected to the first set of metal standoffs and the second set of metal standoffs. The motor can be mounted on the motor plate and operatively connected to one of the plurality of gears.

The subject invention provides systems, apparatuses and methods for cultivating microorganisms and for producing microbial metabolites on a large scale. Specifically, in certain embodiments, a system comprising three separate, but connected, vessels is provided, wherein a first vessel serves as a feed tank for supplying nutrient medium to a second vessel, said second vessel serving as a submerged fermentation reactor; and wherein a third vessel serves as a collection container into which foam containing microbial growth by-products is transferred from the second vessel.

A Disposable Bioprocess System having a Single-Use-Bioreactor, a Single-Use-Pump and a single-use micro-organism retention filter. Combined most suitable for cultivation of suspended micro-organisms in a liquid media at high micro-organism concentration in a perfusion mode continuous process for expression of biological material. The inlet port of the liquid Single-Use-Pump connects via a valve to the broth reservoir of the Single-Use-Bioreactor through a liquid conveying port. The outlet port of the liquid pumping Single-Use-Pump connects via a valve to a micro-organism retention filter. And a method for operating the sterile Disposable Bioprocess System in perfusion mode for continuously processing.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

The membrane bioreactor for simultaneous enzymatic cellulose hydrolysis and product separation is a vessel having a lower hydrolysis reaction chamber and an upper distilled water chamber separated by a semipermeable membrane attached to the top of the lower reaction chamber. The membrane is supported on a stainless steel mesh and sealed to the mesh by epoxy glue to prevent leakage. A peristaltic pump is connected to the reaction chamber and maintains a flow of distilled water through the membrane and the upper chamber, the effluent being collected in a beaker or other product collection vessel. The reaction chamber is agitated at a moderate rate by a magnetic stirrer, and the upper chamber is agitated more rigorously by a mechanical stirrer. A thermocouple and temperature controller and a buffer solution, respectively, maintain temperature and pH in the reaction chamber optimal for enzymatic hydrolysis of cellulose.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

The present invention relates to a bioreactor for cell culture. According to an embodiment of the present invention, vibration is provided to a cell culture solution (solution) to facilitate cell separation, and, since the vibration is transmitted to the entire cell culture solution without dead zones, cell separation efficiency is excellent.

A cell-culture bioreactor operative to provide real-time reactor management in the fields of stir control, sparge control, and heat management responsively to wireless sensor feedback to optimize operating conditions to maximize cell-culture yield; and a bioreactor comprising: a vessel, having an open top; a headplate, configured to seal the vessel's open top; and a stirring device, comprising at least two independent stirring elements; wherein each of the stirring elements is configured to independently stir media accommodated within the vessel.