Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

A system and method for making a liquid chemistry treated biopolymer-based fungal mat is described. The method comprises the steps of harvesting a plurality of fresh mycelium material and marking them for identification, then weighing and recording the initial mass of each of the plurality of mycelium material is carried out. A liquid chemical solution using solvent: chemical ratios from 0:100 to 100:0 is prepared. Next, decanting the liquid chemical solution into a vacuum tumbler drum distributed with the mycelium material. Applying vacuum and rotating the vacuum tumbler drum to ensure thorough mixing and refreshing of the liquid chemical solution at the mycelium surface. Vacuuming and rotating the vacuum tumbler drum is repeated and the at least one fungal mat formed is removed from the vacuum tumbler drum. Finally, draining away surface moisture and air drying the at least one fungal mat.

The present invention relates to a particulate material separation assembly. It comprises a filtration membrane and an antifouling device. The antifouling device comprises one or more magnets and a plurality of magnetisable particles. The one or more magnets cause the plurality of magnetisable particles to self-assemble into dynamic bristles, thereby forming a brush. The particulate material separation assembly is particularly useful in the context of micro algae harvesting.

The bioenvironmental simulation device according to an embodiment of the present invention includes at least one mounting unit on which cells to be measured are placed, a rotational force application unit configured to rotate the mounting unit so as to apply a rotational force to the cells to be measured placed on the mounting unit, and a culture liquid flow device through which a culture liquid flows across the mounting unit, wherein the culture liquid flows by the culture liquid flow device so as to apply a shear force to the cells to be measured.

A microorganism sampling device includes: a head part that has a water supply channel to which a water supply pipe for supplying sample water can be connected; a housing part to an upper part of which the head part can be detachably attached; a frame-structured tube; a tubular filter that is arranged within the tubular body; a cup that communicates with the tubular filter and is attached to one end of the tube body; and a cap that has a channel communicating with the tubular filter and is attached to the other end of the tube body. The microorganism sampling device further includes a filter part housed in the housing part, in which a bottom part of the cup is supported by a bottom part of the housing part. The head part is attached to an upper part of the housing part.

The present invention relates to a method for cultivating cells, in particular tissues, comprising a carrier plate unit, which has at least one access opening, at least one cultivation chamber, and at least one channel connecting the access opening to the cultivation chamber.

This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells. The disclosed compositions and method entail making “reagent bundles” comprising many (hundreds of thousands to millions) clonal copies of an editing cassette and delivering or co-localizing the reagent bundles with live mammalian cells such that the editing cassettes edit the cells and the edited cells continue to grow.

The present invention relates to a device for cultivating cells, in particular tissue, comprising a carrier plate unit which has a central axis of rotation, at least one access opening arranged proximally to the axis of rotation, at least one cultivation chamber arranged distally to the axis of rotation, and at least one channel connecting the access opening to the cultivation chamber, and also a method for cultivating cells in a device according to the invention and a method for producing the device according to the invention.

A reactor arrangement for performing, by means of at least one solid reaction member(s), a biological or chemical transformation, or physical or chemical trapping from, or release of agents to, a fluidic media in a continuous process. The arrangement comprises at least one reactor with a cylindrical reaction vessel (11) in which at least one reactor a transformation device (100) has been mounted. The vessel (11) comprises at least one inlet port (30) in the vicinity of its bottom wall (18) and at least one outlet port (40) arranged in the vicinity of its upper end portion. Each inlet port (30) is connected to a fluid supply member (300) configured to be submerged below the fluid surface level in a pool or a pond. The fluid supply member (300) comprises at least one inlet opening (301) configured to continuously supply a fluid from the pool or the pond to the vessel (11). Each outlet port (40) is configured to continuously let out the fluid from the vessel (11) to the pool or the pond via the outlet port (40). Further a method of using the reactor arrangement is provided.