A modular and stacked cell culture system that includes a standalone cell culture subunit with an interior cavity to house a cell culture substrate in a cell culture space, a fluid inlet to supply fluid to the cell culture space, and a fluid outlet to remove fluid from the cavity. The cavity is arranged for fluid to flow in from the fluid inlet, then through the cell culture space, and then out through the fluid outlet. The subunit further includes an alignment feature on at least one of a top and a bottom of the standalone cell culture subunit, wherein the alignment feature aligns with an alignment feature of another standalone cell culture subunit, such that multiple standalone cell culture subunits are stackable.

The purpose of the present invention is to suppress variation in time for cells to be immersed in a peeling liquid when the cells are peeled from culture layers. A multilayer culture vessel 1 has an internal space that is divided by a boundary surface 10 into a culture space 11 on one side and a buffer space 12 on the other side in a direction parallel to a bottom plate 2. The multilayer culture vessel 1 includes at least one intermediate plate 5 that extends in the culture space along the direction parallel to the bottom plate 2 and divides the culture space 11 into a plurality of culture layers 21, wall portions 13 that respectively extend from the bottom plate 2 and the at least one intermediate plate 5 toward a top plate 3 at the boundary surface 10, communication portions 14 that bring the buffer space 12 into communication with the culture layers 21, and a liquid supply/drainage port 6 that is formed in the top plate 3 at a location facing the buffer space 12.

An assembly includes a housing with opposite first and second layers. The first and second layers are spaced apart to define a confined interior space. A semi-permeable membrane is attached to the first layer, the semi-permeable membrane covering a porous area portion of the first layer. An outlet port and an inlet port are in fluid communication with the interior space. The assembly includes a first circulator for circulating a first fluid between the outlet port and the inlet port, and a second circulator for circulating a second fluid along an exterior surface of the semi-permeable membrane. The second circulator includes a fluid duct attached to or integrated within the housing. The fluid duct is isolated from the interior space and is porous to provide fluid access to an exterior surface of the semi-permeable membrane. The semi-permeable membrane forms a barrier allowing exchange of compounds across the membrane.

Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.

A bioreaction container including a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end; a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space; a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube; a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; and an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure.

The invention refers to a flow-promoting device (100; 100; 100″) for performing a biological or chemical transformation, or physical or chemical trapping from, or release of agents to, a fluidic medium. The flow-promoting device (100; 100; 100″) comprises a ferromagnetic material (5) and a retaining structure (1; 1; 1″), the retaining structure having a compartment (9; 9″) defined by a permeable material (11; 11″). The retaining structure (1; 1; 1″) comprises a top wall (3; 3″) and a circumferential side wall (4; 4″), wherein the top wall (3; 3″) and the circumferential side wall (4; 4″) is formed mainly by said permeable material (11; 11″). The compartment (9; 9″) of the retaining structure (1; 1; 1″) is arranged to contain at least one fluid-permeable solid reaction member.

A fluidic device, a fluidic system and a method for developing a cellular starting material into a three-dimensional cellular structure. The fluidic device includes a base body which includes a chamber in which a matrix is received, into which the cellular starting material to be developed can be introduced, and at least two fluid reservoirs. Each fluid reservoir includes a fluid inlet, a fluid outlet and a separating device which is partially permeable to a fluid medium and which separates the associated fluid reservoir from the chamber and forms a common plane interface with the chamber, via which the fluid medium can diffuse into the matrix. When using suitable fluid media, the fluidic device is adapted to form at least one concentration gradient, at least two mutually orthogonal concentration gradients and/or at least two mutually antiparallel concentration gradients in the matrix, each of which are essentially homogeneous or deliberately inhomogeneous in the z-direction over at least a section of the extension of the matrix.

A packed bed bioreactor is fabricated as a monolith entirely using additive manufacturing techniques, also known as 3D printing. Construction of a bioreactor in this manner enables control over the reactor dimensions and properties (such as void volume) as well as the dimensions, shape, and pattern of the media bed. Together these attributes give the end-user control over the size, shape, material, and flow characteristics of the bioreactor.

An apparatus for managing gas bubbles in a bioprocessing system includes a body portion having a underside surface, and an opening in the body portion. The body portion is configured for placement within a bioreactor vessel such that the underside surface of the body portion is disposed in a liquid within the bioreactor vessel. The underside surface of the body portion is configured to divert rising gas bubbles in the liquid towards the opening.

The present disclosure provides a capture assembly optionally for use in an automated sample analyzer. The sample analyzer includes an optical assembly for scanning a sample well of a planar substrate, e.g., a multiwell plate that is loaded onto and secured to the capture assembly to perform an assay, e.g., detection of an analyte in the sample. The capture assembly automatically aligns the planar substrate about a rotational axis of a drive shaft and secures the substrate to the drive shaft to prevent unwanted movement or slippage of the substrate during starting, stopping and rotation of the substrate at varying rotational velocities thereby ensuring the sample well is reliably detected by the optical assembly.