The present invention relates to a reaction device with air-lift type internal circulation which includes: a vertical cylindrical volume (1), more than one draft tube vertical element (2) positioned within the cylindrical volume (1) in such a manner as to form an gap with the walls of said volume, more than one gas distributor (3), each of which is positioned on the bottom of said device; wherein: each vertical internal element (2) has an internal diameter which increases along the vertical axis of said element, and the ratio between the total height of the reaction device and the internal diameter of the reaction device is less than 1.

This disclosure describes a biochemical reactor with a lower divider support structure. The biochemical reactor may include a tank configured to house immobilized carriers and fluid. The biochemical reactor may include a circulation conduit at least partially disposed within the tank. The circulation conduit may include a circulation outlet opening. The biochemical reactor may include one or more vanes disposed proximate to the circulation outlet opening. The biochemical reactor may include a tank recirculation port disposed proximate to a second end. The biochemical reactor may include a tank inlet configured for feeding fluid into the tank. The biochemical reactor may include a tank outlet configured for drawing fluid from the tank. The tank outlet may be disposed proximate to a first end. The biochemical reactor may include a first divider and a second divider. The second divider may include a support structure including a grating configured to withstand variable loads.

A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths.

Disclosed herein are methods of producing one or more cannabinoid compounds, expanding cells that produce the compounds, cannabinoid compounds produced by the methods, pharmaceutical compositions comprising the cannabinoid compounds, and methods of producing and utilizing the compounds and compositions.

A cell culturing container including a fixed bed is provided for forming a bioreactor (100). In one version, a container in the form of a tubular body is formed by first and second interlocking portions (115a, 115b). The container forms a compartment for receiving the fixed bed (122) for culturing cells, which container may be removably inserted into an inner compartment of the bioreactor so as form an outer chamber for circulating fluid to or from the fixed bed. The container may also include a removable base (130) for receiving an agitator (118) for agitating and thus circulating the fluid. Related methods are also disclosed.

A packed-bed bioreactor is provided that includes: a vessel having an interior cavity defined by an outer wall; and a center column disposed within the interior cavity. The center column includes a columnar sidewall defining an inner region within the center column, the columnar sidewall separating the inner region from an outer region within the interior cavity. The bioreactor further includes a cell culture substrate disposed in the outer region of the cavity, the cell culture substrate surrounding the center column; at least one port extending through the vessel for at least one supply and removal of media to or from the interior cavity; and a fountain head element disposed above the center column.

A syntrophic enrichment for enhanced digestion (SEED) system is presented, in which a retrofit addition to existing anaerobic digestion infrastructure provides improved digestion process rate and biogas quality. The system provides optimal niche environments for accelerating fermentative, syntrophic and methanogenic metabolisms to increase digestion system loading rates and enhance main digester microbiome. Prescribed media formulations, reactor integrations, and operational methods using various fixed and loose media enhance global digestion system performance. The retrofitted system enables existing plants to transition from an outdated solids-management model to one of valorized biomethane production.

A bioreactor for growing micro-organisms, has a reaction chamber containing a reaction mixture with a reaction medium and micro-organisms. A draft tube is arranged inside the reaction chamber, which has a gas inlet, an inlet for the reaction mixture at its first end, and an outlet for the reaction mixture at its second end. The bioreactor includes means for generating flow of the reaction mixture within the reaction chamber and a first blade structure arranged inside the reaction chamber, surrounding the draft tube. The first blade structure has blades arranged at, at least one of an angle α.sub.1 with respect to a direction defined by the height of the reaction chamber, or an angle α.sub.2 with respect to a direction defined by the height of the reaction chamber. The bioreactor also includes an inlet for reaction medium and an outlet for withdrawing medium with grown micro-organisms.

The invention relates to a digester for a liquid medium, comprising a container, a dividing wall between two spaces, and a device for injecting gas into the lower part of one of the spaces. The gas creates an upflow of the mixture of liquid medium and injected gas in said space, and a downflow in the other space. The dividing wall is equipped with at least one device for establishing a fluid communication between the spaces, so that the fluid communication configuration is automatically varied between a first open configuration in which fluid can flow freely from one space to the other, and a second closed configuration in which the flow of fluid is blocked fully or partially by the aforementioned device.

A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths.