An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

A reversible liquid filtration system for cell culture perfusion comprises: a bioreactor vessel (B4), for storing the cell culture (L4); a perfusion pump (P7), comprising a reciprocable element (P71) which is movable in opposing first and second pumping directions (dF, dR); a filter (F4); and first and second bi-directional valves (BV1, BV2), each selectively controllable between open and closed positions. The perfusion pump (P7), the filter (F4), and the first and second bi-directional valves (BV1, BV2), together comprise a fluidic circuit in communication with the bioreactor vessel (B4). The bi-directional valves (BV1, BV2) are controllable to open and close in co-ordination with the reciprocating perfusion pump (P7), in order to enable both a two-way filtering flow around the fluidic circuit and also an alternating filtering flow between the bioreactor vessel (B4) and the perfusion pump (P7).

A device and a method for clinically providing a preparation of autologous phages, namely, those that can verifiably be traced back to originating from a very specific person and preferably are also only intended for use in this one specific person includes a phage culturing device which is a fluid line system that is sealed off with respect to the outside environment The phages in the fluid line system obtained after at least one-time culturing are separated from bacteria by way of filtration, and preferably by way of tangential flow filtration. The phages separated by way of filtration are transferred into a collection vessel that is connected to the fluid line system and are preferably removed from the fluid line system, using the collection vessel, as a usable, preferably autologous preparation.

Embodiments of the present disclosure relate generally to systems and methods for perfusion cell culture involving alternating fluid flows between first and second flexible vessels. For example, a hollow fiber filter module may be attached to first and second culture vessels which each include inner and outer vessels. A pressure source may cause a pressure differential between the outer vessels, which may cause a responsive fluid flow between the inner vessels across a hollow fiber filtration unit.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

A liquid cell culture medium collecting filter unit includes a porous metal membrane that filters out cells in a liquid cell culture medium, a support that holds a peripheral portion of the porous metal membrane. and a tubular member that has a hollow part serving a flow path for a liquid cell culture medium. The tubular member is connected to the support such that the flow path faces at least part of a main surface of the porous metal membrane.

The bioprocessing perfusion system (10) includes a bioreactor (12) and a feed flow path (14). A first tangential flow filter (16) is coupled to the bioreactor (12) via the feed flow path (14) and a second tangential flow filter (18) is coupled to the bioreactor (12) via the feed flow path (14). The first tangential flow filter (16) is a microfiltration-type filter and the second tangential flow filter (18) is an ultrafiltration-type filter. The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to a receiving unit (58) via the permeate flow path (60). The first tangential flow filter (16) and the second tangential flow filter (18) are further coupled to the bioreactor (12) via the retentate flow path (46). A control unit (82) is communicatively coupled to the first feed control device (42), the second feed control device (44), the feed drive unit (40), the first permeate control device (64), the second permeate control device (66), the first retentate control device (48), and the second retentate control device (50).

A waste treatment, pyrolysis and gasification and concerns an apparatus for syngas bio-methanation include a unit for pyrolysis/gasification receiving organic material, the unit for pyrolysis/gasification generating syngas, comprising at least one membrane reactor inside a liquid bath comprising at least one bacteria population, the membrane reactor comprising at least one hollow fiber in contact with the liquid bath, around which a biofilm is formed and into which the syngas from the unit for pyrolysis/gasification flows, so as to convert the syngas into methane. A method for bio-methanation of syngas comprising a step of providing syngas from a unit for pyrolysis/gasification to a membrane reactor inside a liquid bath comprising at least one suitable bacteria population, the membrane reactor comprising at least one hollow fiber in contact with the liquid bath, around which a biofilm is formed and into which the output syngas of the unit for pyrolysis flows, so as to convert the syngas into methane.

A cell culture chamber device for growing cell cultures and tissues. The device includes: an enclosure containing a cell culture media, the enclosure being defined partly by a first end, a second end, and a connecting wall. The first end or a part or window thereof is substantially transparent, and the second end and/or the connecting wall, or a respective part or window thereof, is/are substantially transparent/translucent. The first end is configured to be optically aligned, at least for some period of time or periodically, with the second end and/or with the connecting wall so that light or another illumination or visualisation signal, transmitted through or by the second end and/or through or by the connecting wall into the enclosure is transmitted through the cell culture media and out through the first end to outside the enclosure, and e.g. to outside the cell culture chamber device.