This invention is a system for the production of biomass from photosynthesizing microorganisms that includes a photobioreactor comprising a transparent panel made from two transparent sheets with a separation between them, with top and bottom openings and with transparent, parallel subdivisions that form a panel of vertically arranged, transparent cells, where each transparent cell has a top opening and a bottom opening; a lower recirculation chamber in fluid contact with the bottom openings of the transparent panel; an upper recirculation chamber in fluid contact with the top openings of the transparent panel; a gas distribution tube externally arranged along the edge of said transparent panel; where said gas distribution tube comprises gas injectors arranged in fluid contact with the interior of a plurality of transparent cells; and a supporting structure that supports the transparent panel, the lower recirculation chamber, the upper recirculation chamber and the air distribution tube.

The present technology is generally related to storage structures for cell engineering systems. The storage structures allow for multiple automated cell engineering systems to be held via a single structure, and presented to a user when desired or needed for direct access to the cell engineering system, and then returned to a storage or working position. Many storage structures can be arranged together in a clinical or hospital setting, or other cell therapy engineering manufacturing environment.

The subject invention provides systems, apparatuses and methods for cultivating microorganisms and for producing microbial metabolites on a large scale. Specifically, in certain embodiments, a system comprising three separate, but connected, vessels is provided, wherein a first vessel serves as a feed tank for supplying nutrient medium to a second vessel, said second vessel serving as a submerged fermentation reactor; and wherein a third vessel serves as a collection container into which foam containing microbial growth by-products is transferred from the second vessel.

In preferred embodiments, the subject invention provides two-vessel fermentation systems for producing microbe-based products comprising fungal mycelia and/or spores, and/or bacterial endospores, wherein the systems comprise both a submerged fermentation vessel and a solid state fermentation (SSF) vessel. Advantageously use of the two phases improves the efficiency of producing microorganisms by catering to the different requirements for biomass and/or vegetative cell accumulation as well as the requirements for mycelial growth and/or sporulation.

A cell-culture bioreactor operative to provide real-time reactor management in the fields of stir control, sparge control, and heat management responsively to wireless sensor feedback to optimize operating conditions to maximize cell-culture yield; and a bioreactor comprising: a vessel, having an open top; a headplate, configured to seal the vessel's open top; and a stirring device, comprising at least two independent stirring elements; wherein each of the stirring elements is configured to independently stir media accommodated within the vessel.

A perfusion module and a perfusion culture system are provided, including: a bioreaction module, including at least one bioreactor; a perfusion module, including at least one perfusion chamber, each perfusion chamber is connected to a pressure regulating pipe for inflow or outflow of an airflow; when there are multiple perfusion chambers, every two adjacent perfusion chambers are connected to each other; at least one bioreactor, the perfusion module, the filtration module are communicated in sequence, if there are more than two perfusion chambers, all of perfusion chambers are set side by side; a cell culture fluid in at least one bioreactor flows into at least one perfusion chamber, flows out from at least one perfusion chamber into at least one filter, and a permeate from at least one filter flows into a harvesting device. The present disclosure avoids high shear forces in the perfusion culture system.

The present invention relates to a reactor comprising a vessel (1) for containing: • a mass to be treated, and • at least one lighting device (2a, 2b) intended to promote the treatment of said mass, characterized in that each lighting device (2a, 2b) comprises a light diffuser including at least one micro-etched plate (211) which is transparent to light radiation.

Provided are a culture apparatus and a culture method with which microalgae can be cultured satisfactorily. The culture apparatus includes a plurality of culture tanks and a water storage tank, and cultures microalgae in a culture solution. Each of the plurality of culture tanks has a translucent accommodating portion containing the culture solution and microalgae. The volumes of the accommodating portions of the plurality of culture tanks are different from each other. The water storage tank has a translucent water storage unit for storing a stored water. The plurality of culture tanks are selectively arranged in the water storage unit.

The present invention is a manufacturing method for producing fermentation products using a fermentation vessel, including steps of: preparing a fermentation vessel and a sensor, introducing liquid into the fermentation vessel, and operating the fermentation vessel in which properties of liquid in the fermentation vessel are measured to adjust operating conditions; wherein the sensor device has a sensor for measuring liquid properties and a sensor cover body; a bottom permeable portion for passing liquid and crystals in the liquid is disposed on the bottom surface of the cover body, and a top permeable portion for passing liquid and crystals in the liquid is disposed on the top surface of the cover body; micropores are respectively formed in the bottom permeable portion and the top permeable portion; and micropores disposed in the top permeable portion are the same or larger than micropores disposed in the top permeable portion.

In a culturing method, microalgae are cultured in a culturing solution while a gas containing carbon dioxide is supplied to the culturing solution. In an ion concentration acquisition step, an acquired value of a concentration of hydrogen carbonate ions in the culturing solution is obtained. In an ion concentration adjustment step, in the case that the acquired value does not reside within a set concentration range that is set beforehand, at least one of a temperature or a pH of the culturing solution is adjusted, whereby the concentration of hydrogen carbonate ions in the culturing solution is adjusted to reside within the set concentration range.