The invention relates to a device for isolating stem cells from fetal tissues, which device has an incubation chamber, at least one pump, at least one reservoir for a tissue break-down solution, at least one reservoir for a rinsing solution, optionally a control unit, optionally a means for removing contaminants, and optionally a means for expansion of the isolated stem cells. The invention further relates to a method for isolating stem cells from fetal tissue, which method comprises, among other things, the mechanical dissociation and the enzymatic digestion of the fetal tissue and optionally density gradient centrifugation for removing contaminants. The device and the method according to the invention are particularly suitable for isolating mesenchymal stem cells from fetal tissues, such as umbilical cord tissue, placenta tissue, or fetal lung tissue.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.

A dry ice freeze-pulverizer includes pulverizing container and pulverizer main body. The pulverizing container includes a container main body that includes a bottom plate and a side plate erected from a peripheral edge of the bottom plate, has an upper surface opening, and is provided with a pulverizing blade therein, and a lid that closes the upper surface opening and has a vent hole. The pulverizer main body includes a drive device configured to rotate the pulverizing blade. The side plate of the container main body is formed with a vacuum double structure that is obtained by evacuating an internal space formed by opposing metal plates.

A dry ice freeze-pulverizer includes pulverizing container and pulverizer main body. The pulverizing container includes a container main body that includes a bottom plate and a side plate erected from a peripheral edge of the bottom plate, has an upper surface opening, and is provided with a pulverizing blade therein, and a lid that closes the upper surface opening and has a vent hole. The pulverizer main body includes a drive device configured to rotate the pulverizing blade. The side plate of the container main body is formed with a vacuum double structure that is obtained by evacuating an internal space formed by opposing metal plates.

A method for dissociating cell aggregates in an agitated reactor. The method comprises providing a cell culture comprising cell aggregates in the agitated reactor, contacting the cell aggregates with a dissociation reagent, generating a dissociation force in the agitated reactor and exposing the contacted cell aggregates to the generated dissociation force under conditions sufficient to dissociate the contacted cell aggregates. The method may be used in a process for passaging cells and/or generating dissociated differentiated cells from stem and/or progenitor cells.

A method for dissociating cell aggregates in an agitated reactor. The method comprises providing a cell culture comprising cell aggregates in the agitated reactor, contacting the cell aggregates with a dissociation reagent, generating a dissociation force in the agitated reactor and exposing the contacted cell aggregates to the generated dissociation force under conditions sufficient to dissociate the contacted cell aggregates. The method may be used in a process for passaging cells and/or generating dissociated differentiated cells from stem and/or progenitor cells.

The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

A device for mechanical fragmentation of tissues facilitates preparation of the composition of isolated cells from a tissue sample, the preparation including mechanical fragmentation of the tissue, the device including a motorized support and a container, the motorized support including a motor having a rotor secured to a coupling member, the container internally including a rotary shaft with radial arms sweeping the inside of the container as the shaft rotates, the motorized support and the container including a complementary securing unit that allow the container to be immobilized on the motorized support, the coupling member and the rotary shaft being configured in such a way as to allow axial rotation of the rotary shaft when the rotor of the motor is turning. The radial arms are of two types: rigid vane-type arms, and flexible filamentary-type arms, notably made of nylon or of metal. A corresponding method is also disclosed.

Devices and methods for the efficient disaggregation of tissue samples, separating the tissue into individual intact cells or small aggregates of cells for analysis. A device may include a chamber to receive fluid and a tissue specimen containing more than one cell to be disaggregated. The chamber may include an opening and an agitator in fluid contact with the fluid and the tissue specimen. The agitator may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The device may include an electrical energy source electrically coupled to the micromotor to rotate the impeller sufficient to disaggregate the one or more individual cells from the tissue specimen and in a manner which does not lyse the one or more individual cells.