The present invention relates to a method of changing culture medium of a suspension culture, the suspension culture comprising cells suspended in the culture medium, the method comprising: (i) transferring a fraction of the suspension culture into a container, wherein the container comprises at least one opening at the bottom; (ii) allowing the cells comprised in the fraction of the suspension to settle at the at least one opening at the bottom of the container by gravitation, thereby forming a supernatant; (iii) dispensing the cells settled at the bottom of the container (back) into the suspension culture; (iv) discarding the supernatant.

There is disclosed a modular anaerobic digestion point-of-waste to renewable energy system. The system is directed to a modular and scalable anaerobic digestion system for point-of-waste use. The System includes a pretreatment process for removing inhibitory nutrients from a feedstock, an in-treatment process for providing clean renewable energy and a post-treatment process for further providing clean renewable energy for subsequent use. The System includes a leaching bed; a liquids tank; a mixing tank; an anaerobic digester reactor; a precipitation tank; a stripping tank; a hydrogen sulfide scrubber; a water remover; a gas bladder; a dewaterer; and a flare system.

Separating apparatus, comprising a sedimentation settler and a collection vessel disposed underneath and being in fluid communication with the sedimentation settler, the collection vessel forming a receiving chamber having an outlet at or adjacent to the chamber bottom and having an inlet opening, wherein the collection vessel is arranged such the flow direction of the fluid in the area underneath the sedimentation settler is substantially in line with the direction of the channels of the sedimentation settler.

Disclosed is a method of constructing a cell sheet using only cells without a support. More particularly, a method of manufacturing a multi-layered cell sheet without a separate lamination step and a cell sheet manufactured by the method are disclosed.

The present disclosure relates to a device for liquid processing in the process of cell culture, comprises: a settling tank which is configured to store a cell culture fluid from a bioreactor; a communicating tube that allows the bioreactor to communicate with the settling tank; a first excretory tube for discharging a cell concentrate, which is connected to the settling tank; a second excretory tube for discharging a supernatant, which is connected to the settling tank; a pump; and a control unit. In such device, after the cell culture fluid is separated into the supernatant and the cell concentrate by the gravitational settling in the settling tank, the control unit is configured to control the pump to (a) return the supernatant to the bioreactor through the communicating tube and harvest the cell concentrate through the first excretory tube and/or (b) harvest the supernatant through the second excretory tube and return the cell concentrate to the bioreactor through the communicating tube.

A cell culture device (200) includes a cell culture vessel (22) for culturing cells, a factor container (81) for storing a factor, a reagent container (82) for storing a reagent for introducing the factor into the cells, and a flow path for transporting the factor and the reagent from the factor container (81) and the reagent container (82) to the cell culture vessel (22).

The present invention relates to methods and systems for performing perfusion cell culture whereby the supernatant of the bleed stream is recovered.

Settling or settler devices for separating particles from a bulk fluid with applications in numerous fields. The devices include inclined sliders with an arcuate cross-section arranged in a space within a bioreactor. These devices are useful for separating small (millimeter or micron sized) particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, plant, insect or algal) cell cultures, solid catalyst particle separation from a liquid or gas and wastewater treatment, organic and aqueous phase emulsion separations, or the like.

A microorganism collection device comprises: (a) a base, the base comprising (i) a reservoir comprising a bottom and a side wall extending up from the bottom, (ii) a culture medium within the reservoir, the culture medium disposed upon the bottom of the reservoir and comprising a top surface that is elevated from the bottom higher than the side wall of the reservoir, (iii) a first tab extending laterally outward relative to the reservoir; and (b) a lid comprising (i) an associated state where the lid is covering the culture medium and (ii) a dissociated state where the lid is separated from the base and not covering the culture medium.

The present invention relates to a low specific gravity microcarrier-based cell culture apparatus and a method of monitoring cell culture using the same. More particularly, the present invention relates to four-dimensional cell culture that enables monitoring of the growth state of cells by reflecting a time factor in three-dimensional cell culture and is capable of stable cell expansion culture by minimizing change in cell characteristics.