This disclosure relates to equipment utilized to manufacture chemical agents, particularly biopharmaceuticals, using Disposable Containers (DCs).

Methods for the continuous production, capturing and purification of biologics such as recombinant proteins are described. Also described are pharmaceutical compositions comprising such biologics, as well as methods of treatment and uses of such biologics.

A method for filtering a gas includes passing a gas into a compartment of a casing that includes a polymeric film, the casing having: a first sleeve having a first outlet opening, a first filter being at least partially disposed within the first sleeve so that gas passing through the first sleeve must pass through the first filter; and a second sleeve having an second outlet opening, a second filter being at least partially disposed within the second sleeve so that gas passing through the second sleeve must pass through the second filter, the second sleeve being closed so that the gas passing into the compartment of the casing passes through the first sleeve and the first filter and directly contacts at least a portion of the first sleeve comprised of the polymeric film but does not pass through the second sleeve. Opening the second sleeve when a predetermined condition is met so that the gas passes through the second sleeve and the second filter.

A treatment apparatus for treating biological cell cultures, located in respective storage chambers of a carrier, includes a work deck with one or more bearing spots for bearing the carrier. Each of the bearing spots includes a bearing region corresponding to an area of the carrier. The treatment apparatus additionally includes nozzles for producing a gas flow in a space above the work deck. To avoid liquid droplets or cell material being transported from one storage chamber to another as a result of a horizontal gas flow above the carrier during the treatment, the nozzles are arranged outside of and along an edge of the bearing region or within the bearing region and form a gas curtain around the carrier or the respective storage chambers.

A microscope system includes: an enclosed sample chamber for receiving a sample carrier in an examining position in which a sample arranged on the sample carrier is microscopically examinable; an enclosed incubation chamber that is separated from the sample chamber and that receives the sample carrier in at least one storing position; and a sample carrier transfer unit including an enclosed transfer chamber that is connected to the sample chamber by a first opening, and to the incubation chamber by a second opening, and a sample carrier handling device arranged within the transfer chamber for moving the sample carrier between the storing position and the examination position.

The purpose of the present invention is to efficiently dispense cells while reducing the risk of incorporating bacteria, etc. A cell dispensing device 1 includes: a first tube 23a which connects a container 21 and a dispensing container 22; a syringe pump 24 attached to a second tube 23b connected to a branching portion 31 formed on the first tube 23a; and a buffer tank 25 provided between the syringe pump 24 and the branching portion 31. In addition, a cell suspension contained in the container 21 is temporarily stored in the buffer tank 25 by means of the syringe pump 24, and the cell suspension stored in the buffer tank 25 is delivered to the dispensing container 22.

The invention relates to a single-use connection device for insertion into a connection opening of a bioreactor, in particular in a connection opening of a top plate of a dimensionally-stable bioreactor or in a connection opening of a connection element of a pouch bioreactor. The single-use connection device is inserted in a connection opening of a top plate of a bioreactor and/or in a connection opening of a pouch reactor and is provided with multiple passages and an attachment section, wherein the single-use connection device is formed as a single piece and is made of plastic. The attachment section, on an outer peripheral surface, has an attachment structure for attaching the single-use connection device in the connection opening.

A method for filtering a gas comprises passing a gas through a compartment of a filter assembly, the filter assembly comprising: an inlet opening; a first outlet opening; a casing comprising polymeric film and bounding the compartment, the compartment communicating with the inlet opening and the first outlet opening; and a first filter at least partially disposed within the compartment. The method further comprising forming a first seal across a first section of the casing at a location between the inlet opening and the first filter to form a first sub-compartment within the casing and severing the casing at a first location.

A liquid filtration system comprising a syringe pump comprising a gas chamber and a movable plunger, wherein the gas chamber has an aperture at a first end and the plunger forms a seal within the internal walls of the chamber; a liquid chamber having two openings, the openings positioned at opposite ends of the chamber and the first opening connected to the aperture of the gas chamber; a bioreactor in fluidic communication with the second opening of the liquid chamber; a filter arranged to filter liquid passing between the bioreactor and the liquid chamber, the filter comprising a permeate outlet for removing filtered liquid; wherein, in use, the plunger may be moved in a reciprocating motion causing a corresponding movement of gas which drives liquid alternately between the liquid chamber and the bioreactor such that liquid passes through the filter and filtered liquid may be removed via the permeate outlet.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.