This present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilization module for storing disaggregated product material, wherein each of said modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of said modules comprises one or more ports to permit aseptic input of media and/or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and/or enrichment and/or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method.

A method for improving a biogas production is provided in which an organic substrate is pretreated by various methods. In particular, the method includes a combination of a magnetic and an enzymatic pretreatment of the substrate with an attractive specific energy gain. The application of a magnetic field induces changes in biological systems

The invention relates to a method and an apparatus for producing a soluble carbohydrate containing fraction (10), in which lignocellulose material (3) formed by treating plant based raw material (1) is conducted into a separation stage (4). The method comprises at least one solid-liquid separation stage (4) for separating a soluble carbohydrate containing fraction (10) and/or a washing filtrate (12) from lignocellulose material (3), and at least a part of the soluble carbohydrate containing fraction (10) and/or the washing filtrate (12) is recirculated to the lignocellulose material (3) for increasing concentration of the soluble carbohydrate containing fraction, and solids (11) and at least a part of the soluble carbohydrate containing fraction (10) are supplied out from the separation stage. Further, the invention relates to the soluble carbohydrate containing fraction and the solid fraction, and their uses.

The invention relates to a device for isolating stem cells from fetal tissues, which device has an incubation chamber, at least one pump, at least one reservoir for a tissue break-down solution, at least one reservoir for a rinsing solution, optionally a control unit, optionally a means for removing contaminants, and optionally a means for expansion of the isolated stem cells. The invention further relates to a method for isolating stem cells from fetal tissue, which method comprises, among other things, the mechanical dissociation and the enzymatic digestion of the fetal tissue and optionally density gradient centrifugation for removing contaminants. The device and the method according to the invention are particularly suitable for isolating mesenchymal stem cells from fetal tissues, such as umbilical cord tissue, placenta tissue, or fetal lung tissue.

The invention relates to a method and an apparatus for treating plant based raw material with an enzymatic hydrolysis, in which the plant based raw material (1) is treated to form lignocellulosic material (3a,3b) and the lignocellulosic material (3a,3b) or its fraction (10) is conducted into the enzymatic hydrolysis (4), wherein the method comprises at least one treatment stage (2a,2b,2c) in which the plant based raw material (1) is treated so that the lignocellulosic material (3a,3b) contains over 80% fine solid particles which are fiber-like or indefinable particles smaller than 0.2 mm, defined by an optical measurement device, the lignocellulosic material (3a,3b) or at least one fraction (10) of the lignocellulosic material is supplied into the enzymatic hydrolysis (4) for forming a lignin based material (5), and at least one solid-liquid separation stage (6) after the enzymatic hydrolysis (4) in which a lignin fraction (7) and a soluble carbohydrate containing fraction (8) are separated. Further, the invention relates to the soluble carbohydrate containing fraction, the lignin fraction, the lignin based material, the liquid fraction and the solid fraction, and their uses.

The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.

The invention relates to a method for treating biomass (2). Biomass (2) is fed to a pressurized prehydrolysis reactor unit (8) by means of a feeding system (5, 7), wherein by means of the feeding system (5, 7) the biomass (2) is compressed. A filtrate is squeezed out of the biomass (2) by means of the feeding system (5, 7), in particular by a first plug screw (5) or a second plug screw (7) of the feeding system (5, 7). The biomass (2) is then thermally treated in the pressurized prehydrolysis reactor unit (8), discharged from the pressurized prehydrolysis reactor unit (8) afterwards, diluted with the filtrate before or after the discharge, and treated with an enzyme subsequently.

The present invention relates to an improved method and device for treating biomass in which thermally treated biomass is discharged from a pressurized reactor and introduced into a blow tank, wherein the absolute pressure in the blow tank is maintained below atmospheric pressure. The slurry of biomass separated in the blow tank is then enzymatically treated.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biofuel production. In particular, a sugar/carbohydrate stream, which includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose (aka glucose) and/or has had removed therefrom an undesirable amount of unfermentable components, can be produced after saccharification and prior to fermentation (or other sugar conversion process), with such sugar stream being available for biofuel production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein, oil and/or fiber, prior to fermentation or other conversion systems. In other words, sugar stream production and/or grain component separation occurs on the front end of the system and method.

Embodiments of the present disclosure involve systems and methods that inhibit the production of lactic acid during propagation of yeast and/or during hydrolysis of cellulose by including a sufficient amount of dissolved oxygen.