This present invention concerns a single use aseptic kit comprising: a disaggregation module for receipt and processing of material comprising solid mammalian tissue; and a stabilization module for storing disaggregated product material, wherein each of said modules comprises one or more flexible containers connected by one or more conduits adapted to enable flow of the tissue material there between; and wherein each of said modules comprises one or more ports to permit aseptic input of media and/or reagents into the one or more flexible containers. The invention further relates to an automated device for semi-automated aseptic disaggregation and/or enrichment and/or stabilisation of cells or cell aggregates from mammalian solid tissue comprising a programmable processor and the single use aseptic kit. The invention further relates to a semi-automatic aseptic tissue processing method.

A specimen holding and positioning apparatus operable to substantially non-movably maintain a specimen (e.g., an excised tissue specimen) in a fixed or stable orientation with respect to the apparatus during imaging operations (e.g., x-ray imaging), transport (e.g., from a surgery room to a pathologist's laboratory), and the like for use in facilitating accurate detection and diagnosis of cancers and/or other abnormalities of the specimen.

In some embodiments, an engineered tissue construct (ETC) transport and containment method is provided and includes storing a bioreactor and an ETC contained therein within a transport package via an opening in the package, and sealing the opening. Some embodiments are also directed at methods for sterilizing packaging and bioreactors contained therein.

Vial assemblies comprising a tubular body and a flexible bottom or flexible liner are described herein, wherein the flexible bottom or liner is configured to compress at least partially upon the application of force. Methods for storing and removing frozen samples from such vial assemblies are also described herein.

The disclosure provides methods and compositions for the preservation of bacteria. Aspects of the present disclosure provide methods of preparing a preserved bacterial composition comprising flash freezing a bacterial composition and lyophilizing the flash frozen bacterial composition to produce a preserved bacterial composition. In some embodiments, the bacterial composition comprises one or more bacterial strains. In some embodiments, the one or more bacterial strains comprise one or more anaerobic bacterial strains. In some embodiments, the anaerobic bacterial strains are strict anaerobic bacteria.

The present invention aims to provide a device for cryopreservation which enables easy and reliable cryopreservation of a cell or tissue. The device for cryopreservation of a cell or tissue of the present invention includes a deposition part on which a cell or tissue is to be deposited together with a preservation solution, wherein a surface of the deposition part includes a protrusion for holding a cell or tissue and a recess for storing a preservation solution.

A system for an automated production of high cell density seed culture, HCDC, preparations, for cryo-preservation comprising a cell expansion device for growing a HCDC in a medium inside a device volume of the cell expansion device, at least one sensor for collecting data by at least temporally monitoring at least one parameter being indicative of the physiological state of the HCDC; and a control unit for processing the collected data to determine whether at least one predetermined criterion is fulfilled by the monitored parameter to control, based on whether the at least one predetermined criterion is fulfilled by the monitored parameter, at least a partial exchange of the medium by a cryo-protective agent, CPA.

Provided is a cryopreservation jig fixture including: a base having an upper surface, side surfaces, and a bottom surface; and either a slit on the upper surface of the base or an insertion portion for inserting a cryopreservation jig on the upper surface of the base and a slit having one end opened at a lateral portion of the insertion portion. Also provided is a method of freezing and thawing a cell or tissue, which uses the fixture.

An object of the present invention is to provide a container for cryopreservation and transportation which is excellent in maintainability and can appropriately control the temperature of an object to be frozen. The present invention provides a container for cryopreservation and transportation (1A) used to transport an object to be frozen, including: a thermal insulation container (2) having an upper opening (2a); a thermal insulation lid (3) which closes the upper opening (2a) of the thermal insulation container (2); and a cooling unit (4) which is held in the thermal insulation container (2) while absorbing liquid nitrogen, wherein a housing space (K) for accommodating a storage tool (50) for storing the object to be frozen is provided inside the thermal insulation container (2), and the cooling unit (4) is detachable through the upper opening (2a) of the thermal insulation container (2) while the storage tool (50) located in the housing space (K) is housed in the thermal insulation container (2).

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.