Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

The present invention relates to a method of separating cultured cells, which is used in a configuration including: a first accommodation space; a cell suspension which is present in a liquid in the first accommodation space in a state in which a plurality of cells is connected to one another; a filter container which is hermetically connected to the first accommodation space and mounted with a cell dividing member having one or more through holes having an average diameter of 5 μm to 300 μm; and a second accommodation space which is hermetically connected to the filter container, in which the cell suspension flows from the first accommodation space to the second accommodation space through the filter, such that the connection between the cells is passively and mechanically separated while the cells collide with a fixed resistance portion of the filter.

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation/purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

A cell strainer causes fluid to flow out in a tube via a filter or causes fluid in the tube to flow in via the filter by an operation of a pipette engaged with the tube. The filter is formed into a shape such that the filter is entirely positioned in the tube when the cell strainer is engaged with the tube. A tip engaging hole press-fitted to a distal end of a tip for pipette is formed in the cell strainer. A flow passage communicating with the tip engaging hole is formed inside the cell strainer. The filter has a plurality of openings having similar shapes. The openings communicate between an inside of the flow passage and an internal space of the tube to filter out a substance larger than the openings among substances in the fluid.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

A method for producing a plant life enhancing product is described. The method includes: (i) anaerobically digesting agricultural residue to produce an anaerobically activated organic residue and an ammonia-enriched water; (ii) aerobically digesting the ammonia-enriched water to produce an aerobically activated organic residue; and (iii) blending the anaerobically activated organic residue with the aerobically activated organic residue to produce the plant life enhancing product.

Disclosed is an ultrahigh-pressure homogenizing integrated device and a cell disruptor. The ultrahigh-pressure homogenizing integrated device includes a long oil cylinder, a main connecting sleeve, a high-pressure cylinder homogenizing main body, an auxiliary connecting sleeve and a short oil cylinder, which are sequentially and coaxially arranged. An upper part of the high-pressure cylinder homogenizing main body is provided with a feeding hole communicated with a high-pressure cavity; and the feeding hole is connected with an integrated feeding device. A pressurizing plunger rod in the high-pressure cavity of the high-pressure cylinder homogenizing main body is connected with a piston rod of the long oil cylinder; and a homogenizing valve arranged in the inner cavity, which is communicated with the high-pressure cavity, of the high-pressure cylinder homogenizing main body, is connected with an ejector rod of the short oil cylinder.

Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

A method for producing a plant life enhancing product is described. The method includes: (i) anaerobically digesting agricultural residue to produce an anaerobically activated organic residue and an ammonia-enriched water; (ii) aerobically digesting the ammonia-enriched water to produce an aerobically activated organic residue; and (iii) blending the anaerobically activated organic residue with the aerobically activated organic residue to produce the plant life enhancing product.