A system and method for the production of microbial consortiums and by-product material is provided. A physical containment system comprising phase spaces arranged in a discrete order to favor specific biological reactions is also provided. Phase profiles and phase data sets include the pre-determined physical and biological parameters for the phase space transitions. Movement of material from one phase to the next is hydraulically balanced enabling working fluid to continuously move in a fixed direction and rate of flow. Continuous monitoring of phase profiles and phase data sets provide feedback to the system enabling alteration of the conditions in the system to control reactions therein.

The present disclosure generally relates to a method and a system for forming a storage stable hydrolysate from a lignocellulosic material and to a hydrolysate formed by such a method. It also relates to the use of the hydrolysate to reduce and/or control microbial contamination during storage and/or fermentation. Additionally, the present disclosure relates to a method and a system for reducing and/or controlling microbial contamination in a separate hydrolysis and fermentation (SHF) process.

A system and method for the production of microbial consortiums and by-product material is provided. A physical containment system comprising phase spaces arranged in a discrete order to favor specific biological reactions is also provided. Phase profiles and phase data sets include the pre-determined physical and biological parameters for the phase space transitions. Movement of material from one phase to the next is hydraulically balanced enabling working fluid to continuously move in a fixed direction and rate of flow. Continuous monitoring of phase profiles and phase data sets provide feedback to the system enabling alteration of the conditions in the system to control reactions therein.

A process for purifying a composition comprising water, a target substance, impurities and optionally cells, the process comprising the steps (A) and (B): (A) preparing a liquid feedstock by performing step (Ai) and/or (Aii) on the composition: (Ai) removing at least some of the cells from the composition; (Aii) concentrating the composition by removing water therefrom; and (B) passing the liquid feedstock through an apparatus comprising at least two processing units, each such unit producing a product stream containing purified target substance and optionally a waste stream comprising at least some of the impurities, wherein each unit comprises specified components (i) to (v). The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

A filtration system for downstream processing of a suspension containing a labile solid material in a liquid medium, wherein dissolved gas in liquid environment is controlled to ensure optimal process conditions. The dissolved gas can be controlled by a gas-liquid interface by bubbling; a liquid-liquid interphase transfer process using a gas vector substance; a liquid-solid interphase transfer process using a gas permeable solid or a reaction evolving oxygen or other gas/gases. The retained solid material is removed in batch operations, continuous operations or mixed-type operations. The process can also include further filtration, heat-treatment, and removal of at least a part of the liquid. The filtration system could also process a concentrated suspension which is devoid of the free liquid, then heat-treated and the liquid produced by the treatment removed by further filtration. The solid material can be a biomass.

The invention relates to devices, methods and systems for collecting and solidifying waste material from bioreactor cell cultures in order to facilitate waste disposal. The invention finds particular utility in mammalian cell culture applications. A transverse wall divides the interior of a waste bag into two chambers, the first chamber containing an inlet receiving waste liquid from a bioreactor, the second chamber containing an absorbent material to solidify the liquid into a gel. The transverse wall acts to direct the flow of waste media into the second chamber where it is converted into a gel and further prevents the inlet from being blocked by any gel or particulate materials. Methods and uses regarding this invention are described.

An isolator system 1 includes: a main isolator 3 in which an aseptic state is maintained and which is for performing an aseptic operation; an incubator 4 in which the aseptic state is maintained and which is connected to the main isolator 3 and is for culturing cells and the like; decontamination means 35 for decontaminating the inside of the main isolator 3; and a decontamination station 9 that decontaminates the inside of the incubator 4. The isolator system 1 further includes a blocking member 30 for sealing a connection port 13 from the outside, the port being provided in the main isolator 3 and being for connection with the incubator 4. When the inside of the main isolator 3 is decontaminated, the connection port 13 is sealed from the outside with the blocking member 30. The isolator system that can efficiently decontaminate the main isolator and the subisolator connected to this main isolator can be provided.

A facility for the recycling of biomaterial with a fermentation stage, whereby the fermentation stage has a fermentation chamber for the production of biogas through anaerobic fermentation of the biomaterial, and with a hygiene stage that is positioned downstream from the fermentation stage, whereby the hygiene stage has a hygienization chamber for the reception and the thermal hygienization of biomaterial discharged from the fermentation stage. A process for the recycling of biomaterial by zymosis is also provided, whereby biogas is produced in a fermentation stage through anaerobic fermentation of the biomaterial, whereby the biomaterial is, after flowing through the fermentation stage, conveyed to a hygiene stage in which the biomaterial thermal is hygienized, and whereby the biomaterial is, after flowing through the hygiene stage, made available as recyclable agricultural, hygienized fermentation residue.

An incubator (1) and its use for cultivating microorganisms and/or for carrying out a chemical reaction are provided. The incubator (1) has a sample vessel holder (3) for insertion of a sample vessel (4), a heating device (5) for changing a temperature in the sample vessel holder (3) and/or in a sample vessel (4) inserted into the sample vessel holder (3), and a sample-inactivating device (6) for inactivation of a sample (7) in a sample vessel (4) that is inserted into the sample vessel holder (3).

The present invention provides a system and a method for the production of cells and/or cell products. The system comprises at least one cell culture unit comprising at least one bioreactor for culturing cells, at least one technical control unit for controlling a cell growth parameters, said technical control unit is at least fluidly connected to the cell culture unit, and at least one air treatment unit for treating ambient air, said air treatment unit is fluidly connected to the cell culture unit. The system is characterized in that the system is autonomous and the bioreactor total volume is at most 1000L.