A nanostructure for isolating or concentrating extracellular vesicles or a pathogen, includes a transferrin family protein linked on magnetic nanoparticles. The nanostructure includes a transferrin family protein, and thus has selectivity for a pathogen or extracellular vesicles capable of binding to the transferrin family protein, and the synthesized nanostructure is positively (+) charged. The nanostructure includes magnetic nanoparticles, a target material is easily and simply isolated from other materials by magnetism when a magnetic field is applied.

Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

A liquid cell culture medium collecting filter unit includes a porous metal membrane that filters out cells in a liquid cell culture medium, a support that holds a peripheral portion of the porous metal membrane. and a tubular member that has a hollow part serving a flow path for a liquid cell culture medium. The tubular member is connected to the support such that the flow path faces at least part of a main surface of the porous metal membrane.

A liquid cell culture medium collecting filter unit includes a porous metal membrane that filters out cells in a liquid cell culture medium, a support that holds a peripheral portion of the porous metal membrane. and a tubular member that has a hollow part serving a flow path for a liquid cell culture medium. The tubular member is connected to the support such that the flow path faces at least part of a main surface of the porous metal membrane.

The present relates to a yeast strain and use thereof and a preparation method of ergothioneine. The present invention relates to the field of biotechnology. The yeast strain is obtained through traditional mutagenesis and screening, and its deposit number is CCTCC M 20211505. The present invention provides a preparation method of ergothioneine. The preparation method of ergothioneine comprises: mixing the aforementioned yeast strain with a fermentation medium and an optional substrate, fermenting, and then homogenizing cells and separating to obtain ergothioneine. The aforementioned yeast strain can be used for the preparation of ergothioneine, and the ergothioneine prepared by the yeast strain has the advantages of high yield, low cost and fast preparation speed. The preparation method has the advantages of low cost, environmental protection, high product quality, high yield, less impurities, less drug residues, short fermentation period and the like.

The present relates to a yeast strain and use thereof and a preparation method of ergothioneine. The present invention relates to the field of biotechnology. The yeast strain is obtained through traditional mutagenesis and screening, and its deposit number is CCTCC M 20211505. The present invention provides a preparation method of ergothioneine. The preparation method of ergothioneine comprises: mixing the aforementioned yeast strain with a fermentation medium and an optional substrate, fermenting, and then homogenizing cells and separating to obtain ergothioneine. The aforementioned yeast strain can be used for the preparation of ergothioneine, and the ergothioneine prepared by the yeast strain has the advantages of high yield, low cost and fast preparation speed. The preparation method has the advantages of low cost, environmental protection, high product quality, high yield, less impurities, less drug residues, short fermentation period and the like.

A cell culture substrate includes: a first layer that includes a first gel in which gold nanoparticles dispersed; and a second layer that includes a second gel in which the gold nanoparticles are not present or are present in a lower concentration in comparison with the first layer.

A system for reducing total dissolved solids in wastewater can include a vertical reactor that can include a flexible sheet material, where the flexible sheet material can be configured to facilitate the growth and attachment of an algal biofilm. The vertical reactor can include a shaft, where the shaft can be associated with and can support the flexible sheet material, and a drive motor, where the drive motor can be coupled with the shaft such that the flexible sheet material can be selectively actuated. The system can include a fluid reservoir containing a portion of wastewater through which the flexible sheet material is configured to pass as well as a stressor operably configured to stimulate the algae to produce an extracellular polymeric substance. A method of reducing total dissolved solids in wastewater includes moving an algal biofilm through the wastewater and moving the algal biofilm through a gas.

The present invention relates to an improved diatomaceous earth composition containing salt water. The diatomaceous earth composition according to the present invention comprises an agglomerated mixture of calcined diatomaceous earth particles, water and at least one inorganic salt, wherein the mass ratio of the calcined diatomaceous earth particles and water is in the range of 1:1.0 to 1:2.0, and wherein the content of the at least one inorganic salt is equal to or more than 0.25 parts by mass based on 100 parts by mass of water. In a further aspect, the present invention relates to a method for producing the diatomaceous earth composition according to the present invention. In another aspect, the present invention relates to the use of the diatomaceous earth composition according to the present invention as an agent for precoat filtration or dynamic body feed filtration in biopharmaceutical applications.