The invention provides methods and materials that can be used to determine three dimensional structures of RNA hairpin loops and their complexes with inhibitors easily and quickly. The scaffold RNA, YdaO-type c-di-AMP riboswitch from Thermoanaerobacterpseudethanolicus, readily forms crystals with a large cavity over 60 in diameter. A hairpin of interest can be engineered into the P2 stem of this RNA so that the hairpin is accommodated in the cavity. The fusion RNA is then crystallized, and structures can be determined using X-ray or electron crystallography. Embodiments of the invention can be used to identify compounds that bind hairpin loops in order to, for example, effect therapeutic and other biological activities.

The invention provides methods and materials that can be used to determine three dimensional structures of RNA hairpin loops and their complexes with inhibitors easily and quickly. The scaffold RNA, YdaO-type c-di-AMP riboswitch from Thermoanaerobacterpseudethanolicus, readily forms crystals with a large cavity over 60 in diameter. A hairpin of interest can be engineered into the P2 stem of this RNA so that the hairpin is accommodated in the cavity. The fusion RNA is then crystallized, and structures can be determined using X-ray or electron crystallography. Embodiments of the invention can be used to identify compounds that bind hairpin loops in order to, for example, effect therapeutic and other biological activities.

The invention provides a protein scaffold and methods of preparing, screening, engineering and using the protein scaffold.

A factor that is caused by a nucleic acid and influences the kinetics of an extracellular vesicle is screened. A library of barcoded extracellular vesicles is provided.

The present invention relates to proteins suitable for being used as scaffolds to which a peptide of interest is bound, or which are comprised within a conjugate to which an agent of interest is attached. It also relates to said conjugates suitable for the selective delivery of their conjugated agents of interest to specific cell and tissue types, wherein said agent 5 can be a therapeutic agent or an imaging agent. It also relates to nanoparticles comprising such conjugates and the therapeutic uses thereof.

The present invention provides a fibronectin type III (Fn3) molecule, wherein the Fn3 contains a stabilizing mutation. The present invention also provides Fn3 polypeptide monobodies, nucleic acid molecules encoding monobodies, and variegated nucleic acid libraries encoding such monobodies. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform the methods.

Disclosed are compositions and method related to variants of SPINK2 that bind to targets other than an endogenous target of SPINK2. In one embodiment, a peptide is provided that comprises the amino acid sequence SEQ ID NO: 1. In further embodiments, an amino acid sequences encoded by nucleotide positions 4 to 42 and/or nucleotide positions 94 to 189 in the nucleotide sequence of SEQ ID NO: 14 flank the amino terminus and the carboxyl terminus, respectively, of the amino acid sequence. In another embodiment, a peptide is provided that comprises an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 in which a conservative substitution, deletion, addition and/or insertion of 1 to 5 (inclusive) amino acids has occurred at amino acids other than the 1st Xaa to the 12th Xaa counting from the amino terminus.

The present invention relates to complexes of oligonucleotide-encoded libraries and methods of tagging and using such libraries. In particular, the oligonucleotides and methods can include complexes having at least one linkage for which a polymerase has reduced ability to read or translocate through.

The present invention relates to complexes of oligonucleotide-encoded libraries and methods of tagging and using such libraries. In particular, the oligonucleotides and methods can include complexes having at least one linkage for which a polymerase has reduced ability to read or translocate through.

The present disclosure provides ultrahigh-throughput single cell genomic sequencing methods, referred to herein as “SiC-seq”, which methods include encapsulating single cells in molten gel droplets to facilitate bulk cell lysis and purification of genomic DNA in microgels. Systems and devices for practicing the subject methods are also provided.