Compositions and methods for transforming cells and generating transgenic grapevine plants comprising two silencing suppressor proteins C2 and V2 encoded by GRBV and recombinant hairpin vectors targeting C2 and V2, which are be used to generate stably transformed transgenic grapevine plants.

A method according to an embodiment of the present disclosure is for enhancing disease resistance of a plant against Soybean mosaic virus compared to a non-transformant. The method may include transforming a plant cell of the plant with a recombinant vector containing a gene encoding Glycine max purple acid phosphatase 2.1 (GmPAP2.1) protein from Glycine max to overexpress the gene encoding GmPAP2.1 protein. As the GmPAP2.1 gene from Glycine max of the present invention can modulate disease resistance against Soybean mosaic virus, it is expected to be applied for development of new cultivars with enhanced resistance to Soybean mosaic virus to thereby increase soybean productivity.

The invention relates to a Solanum lycopersicum plant that is resistant to TBRFV, which plant comprises a QTL on chromosome 11 between SEQ ID NO: 1 and 53. The presence of the QTL on chromosome 11 is identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 4 to 52. The QTL is as comprised in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42882, NCIMB 42885, NCIMB 42887, or NCIMB 42890.

The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.

The present invention relates to Solanum lycopersicum (S. lycopersicum) plants with resistance to Tomato Yellow Leaf Curl Virus (TYLCV), powdery mildew (PM) and nematodes. According to the invention, the resistances are provided by coupling in cis on the same chromosome the OL4 gene conferring resistance to PM and nematodes and TY1 gene conferring resistance to TYLCV, without coupling the Mi-1 gene conferring resistance to nematodes in cis with said OL4 gene conferring resistance to PM and nematodes and TY1 gene conferring resistance to TYLCV. The genes can be present homozygously or heterozygously in the genome of the S. lycopersicum plants, and they confer resistance to TYLCV, PM and nematodes. The present invention also provides methods for making such plants, and to methods of detecting and/or selecting such plants.

The present invention relates to a modified CCA gene which encodes a CCA-adding enzyme, which modified CCA gene leads to resistance against a positive-strand RNA virus having a transfer RNA-like structure (TLS). The invention further relates to plants and seeds comprising the modified genes, methods for making and identifying such plants and use of the gene.

The present application relates to a compositions and methods comprising or expressing a hevamine A-related MoMo30 protein from Momordica balsamina. The MoMo30 protein is about 30 kDa in size, is stable after being autoclaved at 120° C. for 30 min, resists proteolytic cleavage by trypsin, exhibits mannose-sensitive binding to HIV gp120, exhibits hemagglutinin and chitinase activity, is capable of activating and stimulating T cell proliferation, is capable of preventing infection by HIV-1 or alleviating symptoms in an HIV-1 infected patients, and comprises an amino acid sequence of SEQ ID NO: 4. The MoMo30 protein and/or a nucleic acid encoding the same may be used in methods for preventing or treating microbial infections by HIV, SARS-CoV-2 and other enveloped viruses, as well as other microorganisms comprising cell surface proteins containing glycan residues, such as mannose.

The invention relates to a Solanum lycopersicum plant that is resistant to TBRFV, which plant comprises a QTL on chromosome 11, and/or a QTL on chromosome 12, and/or a QTL on chromosome 6. The presence of the QTL on chromosome 11 can be identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 1, 9, and 2-8; the presence of the QTL on chromosome 12 can be identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 10, 15, and 11-14; and the presence of the QTL on chromosome 6 can be identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 16, 25, and 17-24. The QTL is as comprised in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42879, NCIMB 42880, NCIMB 42881, NCIMB 42882, NCIMB 42883, NCIMB 42884, NCIMB 42885, NCIMB 42886, NCIMB 42887, NCIMB 42888, NCIMB 42889, or NCIMB 42890.

The application concerns melon plants (Cucumis melo) resistant to infection with tomato leaf curl New Dehli virus (ToLCNDV). The resistant melon plants have a genomic introgression fragment on chromosome 5 which confers tolerance to ToLCNDV in a dominant manner. Also disclosed are markers for identifying those fragments, methods for identifying or producing resistant melon plants.

The present invention relates to a modified ABCB9 gene encoding a protein conferring resistance to CMV in a plant of the Cucurbitaceae, such as a cucumber plant, in which the protein is expressed, where the gene has a) a nucleotide sequence which encodes a protein with SEQ ID NO: 4; b) a nucleotide sequence with SEQ ID NO: 3; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein with SEQ ID NO: 4; d) a nucleotide sequence that encodes a protein with an amino acid sequence, which is at least 85% identical to SEQ ID NO: 4; e) a nucleotide sequence which is at least 85% identical to SEQ ID NO: 3; or f) a nucleotide sequence according to c) or d) wherein the protein has a methionine (M) on position 428 of SEQ ID NO: 4, or on a position corresponding thereto.