The present disclosure relates generally to a molecular delivery system and methods of using the molecular delivery system to deliver biomolecules into cells. In particular, the molecular delivery system comprises a nanotube (NT) array comprising a plurality of nanotubes (NTs) which are used to deliver biomolecules to cells. The NTs can be loaded with molecules that can be delivered into cells following contact (e.g. penetration) by the NTs.

The present disclosure relates generally to a molecular delivery system and methods of using the molecular delivery system to deliver biomolecules into cells. In particular, the molecular delivery system comprises a nanotube (NT) array comprising a plurality of nanotubes (NTs) which are used to deliver biomolecules to cells. The NTs can be loaded with molecules that can be delivered into cells following contact (e.g. penetration) by the NTs.

The present disclosure provides for methods of delivering cargo material to a cell(s) in vivo or in vitro, devices configured to deliver cargo material to the cell, systems configured to deliver cargo material to the cell, and the like. The present disclosure can synergistically combine multiple technologies to improve the rate of cargo material introduction and utility of physical cellular modification in a transformative way compared to the current microinjection state-of-the art.

Provided are a microfluidic apparatus, a method and system for introducing a substance into a cell. The microfluidic apparatus includes a cavity channel, a bulk wave generating device and a surface acoustic wave generating device; a microstructure is arranged on an inner wall of the cavity channel, and the microstructure is constructed for forming a bubble by a solution at the microstructure when the solution is injected into the cavity channel; the bulk wave generating device is configured to generate a bulk wave, the bulk wave enables the bubble to resonate for generating a flow field; and the surface acoustic wave generating device is configured to generate a surface acoustic wave and control a position of at least one particle in the solution.

Two or more micropipettes are used to increase a microinjection throughput in an automated system for microinjecting adherent cells on a Petri dish. In the system, a motorized stage carrying the Petri dish sequentially visits the cells according to an optimized injection sequence. The sequence is selected by minimizing a total distance traveled by the motorized stage such that each cell is visited once by one of the micropipettes. Using multiple micropipettes advantageously reduces the minimized total distance over using a single micropipette to thereby increase the throughput. The optimized injection sequence is obtained by solving an equality-generalized traveling salesman problem. Each micropipette is mounted on a motorized micromanipulator. The motorized stage and motorized micromanipulators operate coordinately that each micromanipulator goes down or up during movement of the motorized stage to compensate for unevenness between a focus plane and a moving trajectory of the motorized stage.

Two or more micropipettes are used to increase a microinjection throughput in an automated system for microinjecting adherent cells on a Petri dish. In the system, a motorized stage carrying the Petri dish sequentially visits the cells according to an optimized injection sequence. The sequence is selected by minimizing a total distance traveled by the motorized stage such that each cell is visited once by one of the micropipettes. Using multiple micropipettes advantageously reduces the minimized total distance over using a single micropipette to thereby increase the throughput. The optimized injection sequence is obtained by solving an equality-generalized traveling salesman problem. Each micropipette is mounted on a motorized micromanipulator. The motorized stage and motorized micromanipulators operate coordinately that each micromanipulator goes down or up during movement of the motorized stage to compensate for unevenness between a focus plane and a moving trajectory of the motorized stage.

Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition.

Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition.

The invention provides T cell receptors (TCRs) that bind Hepatitis B virus (HBV) antigens. The present invention also provides methods of producing, screening and selecting the TCRs, therapeutic applications of the TCRs, and libraries of the TCRs.

The invention provides T cell receptors (TCRs) that bind Hepatitis B virus (HBV) antigens. The present invention also provides methods of producing, screening and selecting the TCRs, therapeutic applications of the TCRs, and libraries of the TCRs.