Cells may be cultured by a method including the following steps: a first step of preparing a population of cell aggregates having a major axis of not more than 400 μm, and a second step of suspension culturing the population of cell aggregates obtained in the first step.

Improved production of recombinant proteins in E. coli, reliant on tightly controlled autoinduction, triggered by phosphate depletion in stationary phase. The process also provides an optimized autoinduction media, enabling routine batch production at various culture volumes where cells densities routinely reach ˜5-7 g cell dry weight per liter and offer protein titers above 2 g/L. The methodology has been validated with a set of diverse heterologous proteins and is of general use for the facile optimization of routine protein expression from high throughput screens to fed-batch fermentation.

Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein.

A monocytes derived signaling cells mixture is prepared by culturing buffy coats in a cell culture medium containing platelet-poor-plasma and collecting the cultured buffy coats and the cultured cell culture medium. The monocytes derived signaling cells mixture contains granulocytes, lymphocytes, fibrocytes and monocytes, in which the high ratio of the monocytes are CD16 positive monocytes. The monocytes derived signaling cells mixture is used in the improvement of liver function index of a subject.

Described herein are efficiently dissolving tablets of dry cell culture media, feeds, supplements, media subgroups, buffer concentrates, or media components useful in culturing cells or microorganisms, methods of manufacturing, and methods of use. In particular, formulations of tableted cell culture media feeds, supplements, media subgroups, or buffer concentrates are described.

The present disclosure relates to a production medium for microorganisms converting methanol to an organic acid and a culture process, wherein the converted organic acid is oxalic acid, and the production medium for microorganisms comprises 1 to 5% of methanol, 1 to 5% of xylose, and 0.01 to 0.05% of calcium chloride relative to 1 L of the total medium, and further comprises potassium dihydrogen phosphate (KH.sub.2PO.sub.4), ammonium sulfate ((NH.sub.4).sub.2SO.sub.4), magnesium sulfate (MgSO.sub.4), iron sulfate (FeSO.sub.4), manganese sulfate (MnSO.sub.4), zinc sulfate (ZnSO.sub.4), or boric acid (H.sub.3BO.sub.3). According to the present disclosure, provided is an organic acid production process using microorganisms of the genus Aspergillus (Aspergillus. sp), which enables a high-throughput production of high-value-added value organic acids such as oxalic acid by utilizing methanol obtained as a product from refining Cl gas such as methane.

The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more growth factors, one or more fatty acids, one or more buffer components, selenium and one or more further trace elements and its use in the culture of cancer stem cells, in particular tumorsphere culture of cancer stem cells.

The present invention discloses formulation of a serum-free medium used for human pluripotent stem cells, which comprises the following raw materials: inorganic salt components, organic components, amino acids and amino acid salts, energy substances and metabolic intermediates, vitamins and antioxidants, proteins and polypeptides, trace elements and chromogenic substances; while the culture process comprises the following steps: selecting a basic formulation, performing combination screening, identifying and evaluating results, and testing a new formulation of culture; and proportioning according to the following methods: adding aforesaid raw materials into 950 ml of water for injection, stirring gently until dissolved, and finally adding 2.438 g of sodium bicarbonate, and stirring gently until dissolved, and then adding 1 liter of water for injection, adjusting the pH to the desired value with 1 mol/L sodium hydroxide solution or 1 mol/L hydrochloric acid solution, finally filtering sterilized with 0.1 μm diameter filter under positive pressure, and storing the medium solution in dark place at 2° C.-8° C., the invention solves the problem of high cost of domestic import of serum-free formulation.

This application provides a method for culturing Lactobacillus plantarum, which comprises culturing Lactobacillus plantarum in a medium comprising molasses, a yeast extract and sucrose at a temperature from 32° C. to 37° C.

The present invention relates to a method for producing a recombinant protein in yeast cells, wherein the cells are subjected to a temperature shift at a specific timepoint of the cell culture. It also relates to a method for producing a recombinant protein in yeast cells by culturing said yeast cells in a medium having a high concentration of potassium ions compared to the concentration of sulfate and/or phosphate ions.