Provided are methods for processing a blood related sample comprising: (a) providing a blood related sample comprising one or more target cells, platelet cells, red blood cells; and (b) reducing a number of the platelet cells in the blood related sample while maintaining a ratio of the red blood cells to the one or more target cells above a critical threshold to produce a reduced platelet blood related sample comprising the one or more target cells. Also described herein are cell compositions produced by applying the methods described herein.

The present invention relates to production of autologous genetically engineered T cells for use in cell therapy applications.

A method for continuous in vitro propagation of Babesia microti and microti-like species is disclosed. The method comprises incubating B. microti in culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody. In one embodiment, the source of anti-B. microti IgM antibody is substantially free of IgG antibody. In one embodiment, the source of complement is a cell or cell line that secretes complement.

A method for continuous in vitro propagation of Babesia microti and microti-like species is disclosed. The method comprises incubating B. microti in culture medium comprising host erythrocytes in the presence of a source of complement and a source of anti-B. microti IgM antibody. In one embodiment, the source of anti-B. microti IgM antibody is substantially free of IgG antibody. In one embodiment, the source of complement is a cell or cell line that secretes complement.

The present invention relates to production of autologous genetically engineered T cells for use in cell therapy applications.

Disclosed herein are cell-targeting complexes that are coated on the surface with target specific antibodies for induction of biological stimulus in target cells/tissue/organs. In some embodiments, the cell-targeting complex involves non-nucleated (e.g. platelets, red blood cells (RBC)) or enucleated cells that have been thiolated, streptavidinylated, and then coated with biotinylated antibodies. In some embodiments, the cell-targeting complex involves multilayer alginate hydrogel beads that have been coated with polyanionic proteins using a polycation, which is then thiolated, streptavidinylated, and then coated with biotinylated antibodies.