The present disclosure relates to a bioengineering process to derive hair follicles in vitro from the in vitro disposition and differentiation of autologous pluripotent stem cells and dermal papilla stem cells. The present disclosure also relates to the in vitro bioengineering of hypoimmunogenic hair follicles from allogenic pluripotent stem cells and dermal papilla stem cells. The present disclosure also relates to bioengineering of autologous and allogenic hypoimmunogenic hair follicles and hair follicle containing sheets with asymmetric disposition of hair shafts. The present disclosure also relates to a bioengineering process to derive hair follicle containing sheets in vitro from a biodegradable supportive grid and said in vitro derived hair follicles. The present disclosure also relates to the controlled asymmetry of the hair shaft on said hair follicle containing sheets. The present disclosure also relates to the field of cosmetic materials and method for reconstructing hair follicle containing materials in vitro.

The invention provides a serum replacement, a preparation method therefor, and an application thereof. The serum replacement contains plasma and a platelet lysate derived from a bovine fetus or a newborn calf, and can be prepared by means of carrying out platelet lysis on platelet plasma from the bovine fetus or the newborn calf.

An object of the present invention is to provide a novel technique for easy culture of mesenchymal stem cells from a removed hair. The present invention to solve the above problem is a method of culturing mesenchymal stem cells, and the method includes a culture step of culturing a hair acquired by removal and including a hair bulb covered with a dermal sheath tissue in a culture solution.