Provided is a method for producing an artificial recombinant RNA virus stably expressing a foreign gene, comprising the steps of: (1) obtaining a foreign gene having a modified codon composition similar to that of an RNA virus gene; (2) inserting the foreign gene obtained in step (1) into an RNA virus genome; and (3) artificially synthesizing an artificial recombinant RNA virus using reverse genetics.

The present invention relates to a method for virus propagation. More closely the invention relates to a method for animal component free propagation and production of rotavirus (RV) using recombinant trypsin.

Genetic circuits have been developed to regulate behaviors of replicon RNA in responses to small molecules, which has broader applications, such as for quantitative expression of cargo genes, temporary expression of immunomodulatory cytokines or antigens for better cancer immunotherapy or vaccination, and for increased safety in use of self-replicating vectors or in combination with other viral-delivery vectors. Described herein are genetic circuits suitable for systems that either require a tight off state or a slow off state, which can serve for instance where either a kill switch or prolonged protein expression (e.g., of vaccine antigens) are needed.

The present invention relates to a method for virus propagation. More closely the invention relates to a method for animal component free propagation and production of rotavirus (RV) using recombinant trypsin.

Rotavirus vectors encoding in their genome a heterologous gene, and nucleic acid constructs encoding such rotavirus vectors. The rotavirus vector genome may include a rotavirus non-structural protein, a 2A peptide downstream of the rotavirus non-structural protein, and a heterologous protein downstream of the 2A peptide. The heterologous gene may be, for example, a SARS-CoV-2 spike protein or a fragment thereof, or an RSV F protein or a fragment thereof.

Genetic circuits have been developed to regulate behaviors of replicon RNA in responses to small molecules, which has broader applications, such as for quantitative expression of cargo genes, temporary expression of immunomodulatory cytokines or antigens for better cancer immunotherapy or vaccination, and for increased safety in use of self-replicating vectors or in combination with other viral-delivery vectors. Described herein are genetic circuits suitable for systems that either require a tight off state or a slow off state, which can serve for instance where either a kill switch or prolonged protein expression (e.g., of vaccine antigens) are needed.

Provided is a method for producing an artificial recombinant RNA virus stably expressing a foreign gene, comprising the steps of: (1) obtaining a foreign gene having a modified codon composition similar to that of an RNA virus gene; (2) inserting the foreign gene obtained in step (1) into an RNA virus genome; and (3) artificially synthesizing an artificial recombinant RNA virus using reverse genetics.

Disclosed herein are polynucleotides encoding recombinant rotaviruses (RVs), methods of using the same, and systems for generating the recombinant RVs.