A virus inactivation method is provided. The method includes irradiating a solution containing an artificially expressed antibody or antibody fragment with ultraviolet rays in the presence of a radical scavenger. The irradiating only aggregates 5% or less of the antibody or antibody fragment. A wavelength of the ultraviolet rays is 200 nm or longer and 315 nm or shorter. An irradiation amount of the ultraviolet rays is 300 mJ/cm.sup.2 or more. An irradiation time of the ultraviolet rays is 3 minutes or less. A concentration of the radical scavenger in the solution is 0.03 mM or more.

The present invention discloses a method for terminal inactivation of pathogenic microorganisms, comprising the following steps of: a. vacuum freeze-drying a bio-product packaged in a container, feeding in a gas and sealing to obtain the end product and, b. performing inactivation by dry-heating. The method provided by the present invention can effectively inactivate non-lipid enveloped viruses, with excellent inactivation effects and short inactivation time particularly to parvoviruses, and thus overcome the deficiencies of the conventional terminal dry-heating inactivation methods.

Described is a reproducible, effective and scalable process for parvovirus production including characterization strategies, preferably production of H-1PV.

Described is a reproducible, effective and scalable process for parvovirus production including characterization strategies, preferably production of H-1PV.