The present application relates a composite multi-epitope expression cassette, a recombinant virus composed thereof and application thereof, and in particular to a chimeric recombinant Newcastle disease virus inserted with an IBV epitope cassette and a vaccine prepared by using the virus. The expression cassette comprises: (a) T cell epitopes derived from S1 proteins of avian infectious bronchitis virus Holte strain and avian infectious bronchitis virus QX-like strain; and (b) B cell epitopes derived from S1 protein of avian infectious bronchitis virus Australian T strain. In the present application, the multi-epitope chimeric ST/B gene of avian infectious bronchitis virus is inserted into the backbone of LaSota strain, so that the LaSota strain can express S1-T/B protein. Thus, the purpose of preventing both ND and IB diseases is achieved. In addition, the T cell epitopes and B cell epitopes act synergistically to produce an earlier and more comprehensive immune response against virus.

The present application relates a composite multi-epitope expression cassette, a recombinant virus composed thereof and application thereof, and in particular to a chimeric recombinant Newcastle disease virus inserted with an IBV epitope cassette and a vaccine prepared by using the virus. The expression cassette comprises: (a) T cell epitopes derived from S1 proteins of avian infectious bronchitis virus Holte strain and avian infectious bronchitis virus QX-like strain; and (b) B cell epitopes derived from S1 protein of avian infectious bronchitis virus Australian T strain. In the present application, the multi-epitope chimeric ST/B gene of avian infectious bronchitis virus is inserted into the backbone of LaSota strain, so that the LaSota strain can express S1-T/B protein. Thus, the purpose of preventing both ND and IB diseases is achieved. In addition, the T cell epitopes and B cell epitopes act synergistically to produce an earlier and more comprehensive immune response against virus.

Provided are compositions and methods that involve recombinant Newcastle disease viruses (rNDVs) that contain an S protein of infectious bronchitis virus (IBV). The rNDV particles include a contiguous segment of IBV S protein that spans an IBV cleavage site between IBV SI and IBV S2 proteins, and can include a full length IBV S protein. Because the particles are multivalent they also stimulate a protective immune response against NDV infection. The compositions are particularly useful for use with avian animals, such as chickens. Isolated rNDV particles, and vaccine compositions that contain them are also provided.

A live or inactivated recombinant vaccine is described, comprising a viral vector and a pharmaceutically acceptable vehicle, adjuvant and/or excipient, wherein the viral vector is capable of generating a cell immune response due to an increased alpha and/or gamma interferon production, and is capable of a quick replication, and it has inserted a nucleotide sequence of the ORF 5 and ORF 6 from PRSS.

Provided are compositions and methods that involve recombinant Newcastle disease viruses (rNDVs), and recombinant vectors that encode them. The rNDVs comprise and/or encode a combination of at least two proteins that are hemagglutinin (HA), neuraminidase (NA) protein, matrix 1 protein (M1), or nonstructural 1 protein (NS1). The HA, NA, protein, M1, and nonstructural 1 protein (NS1) are from Avian Influenza virus (AIV). Method are provided and involve administering an immunologically effective amount rNDV to avian animals to stimulate a protective immune response the rNDV administration constitutes a first (prime) immunization, which can be followed by a second (boost) administration with an avirulent NVD that may include/encode an AIV HA. The avian animals can survive challenges from pathogenic and highly pathogenic NVD and AIV.