Provided are methods of making and delivering vaccine compositions using an enucleated cell-based platform. Methods of clearing pathogenic infections in a subject using the enucleated cell-based platform is also provided. Such enucleated cell-based platform reduces the vaccine development timeline as compared with conventional biological vaccines, and improves vaccine efficacy.

An inactivated virus vaccine microneedle product includes a backing and an inactivated virus-containing microneedle array attached to a side of the backing, wherein the inactivated virus-containing microneedle array includes a plurality of microneedles, and each microneedle contains a matrix and an inactivated virus loaded in the matrix. The present invention adopts the inactivated virus vaccine microneedle product as well as a preparation method and an application thereof, and such microneedle product realizes efficient transdermal absorption of a vaccine by loading an inactivated virus in the microneedle product after being administered to the skin, and a long-acting stable release of a vaccine is achieved, which solves the problems of traditional vaccination, such as muscular pain and multiple injections.

This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

A device for capturing momentarily an exhaled breath of a COVID 19 patient containing active SARS-CoV-2 virions within an accessible compartment of said device and converting said active SARS-CoV-2 virions into far-UVC inactivated SARS-CoV-2 virions by exposure to an activated 222 nm far-UVC lamp mounted in said accessible compartment and with the next inhaled breath of said COVID 19 patient said far-UVC inactivated SARS-CoV-2 virions are positioned within the respiratory system ready to be captured by an antigen-presenting cells such as the Dendritic cells (DCs) which are antigen-presenting cells that capture, process, and present antigens to lymphocytes to initiate and regulate the adaptive immune response. Said far-UVC inactivated SARS-CoV-2 virions can be collected from said accessible compartment of said device and processed into viable vaccine that can be administered to front-line workers.

Methods for producing and providing a vaccine for immunizing an individual against an illness caused by a virus are provided, including viruses from the family of coronaviruses. The method includes obtaining a sample of the virus; inactivating the virus by destroying or removing nucleic acids carrying genetic information for the virus; and preparing the inactivated virus in order to obtain an administrable vaccine to be administered. Vaccines provided in such a manner and the use thereof are also provided.

This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

The present invention relates to novel infectious bronchitis virus strains and the uses thereof. The invention particularly relates to an inactivated or attenuated IBV, as well as to vaccine compositions comprising the same and the uses thereof to vaccinate avians. The invention also relates to nucleic acids, infected cells and methods for detecting the infectious bronchitis virus strains of the invention in any sample.

This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

The present invention relates to novel infectious bronchitis virus strains and the uses thereof. The invention particularly relates to an inactivated or attenuated IBV, as well as to vaccine compositions comprising the same and the uses thereof to vaccinate avians. The invention also relates to nucleic acids, infected cells and methods for detecting the infectious bronchitis virus strains of the invention in any sample.

An apparatus and method for deactivating coronavirus from a liquid having entrained coronavirus. The apparatus comprises a nanosecond pulsed electric field (nsPEF) generator which imparts nsPEF energy to the through the solution to the coronavirus. The nsPEF disrupts the viral membrane, deactivating the virus. The apparatus may be static using a reservoir for batch treatment of viral solution, or may be dynamic using a closed loop for continuous treatment of viral solution. Total energy input may be less than 3500 kVns providing an efficient and effective process. The method and apparatus according to the present invention advantageously avoid boiling.