The present disclosure relates to a pharmaceutical composition comprising a nucleic acid molecule of an adjuvant, a metal complex stabilizing the nucleic acid molecule, and optionally an immunogen that may be a peptide or a protein, or a composition of stabilizing the nucleic acid molecule of the adjuvant comprising the metal complex. The metal complex interacts with the nucleic acid molecule of the adjuvant and/or the immunogen so as to stabilize such pharmaceutically active ingredients, and induces continuous effectiveness of the active ingredients without degradation.

The invention relates to a messenger ribonucleic acid (mRNA) molecule lacking a cap molecule, for which the cost of in vitro transcription synthesis is greatly reduced, comprising, from 5 to 3, at least one copy of a GUCAGRYC(N.sub.7-19)GCCA(N.sub.12-19)UGCNRYCUG consensus sequence which is resistant to the Xrn1 exoribonuclease, a copy of an internal ribosome entry site (IRES) RNA sequence, and an open reading frame.

The present disclosure relates to a pharmaceutical composition comprising a nucleic acid molecule of an adjuvant, a metal complex stabilizing the nucleic acid molecule, and optionally an immunogen that may be a peptide or a protein, or a composition of stabilizing the nucleic acid molecule of the adjuvant comprising the metal complex. The metal complex interacts with the nucleic acid molecule of the adjuvant and/or the immunogen so as to stabilize such pharmaceutically active ingredients, and induces continuous effectiveness of the active ingredients without degradation.