A reporter mycobacteriophage comprising a heterologous nucleic acid comprising a promoter-reporter construct encoding a promotor operably linked to a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises a luciferase protein linked to a fluorescent protein. Methods of utilizing the reporter mycobacteriophage in detection of a viable target microbe in a sample, screening a test substance for treatment of tuberculosis, and detecting a drug-resistant Mycobacterium in a subject are provided.

Disclosed herein are methods and compositions for determining the presence or absence of a mechanism of antimicrobial resistance in a sample.

Disclosed herein are methods and compositions for determining the presence or absence of a mechanism of antimicrobial resistance in a sample.

Disclosed herein are methods and compositions for determining the presence or absence of a mechanism of antimicrobial resistance in a sample.

Disclosed herein are compositions and methods that can be used to treat subjects with Wilson's disease. The compositions can comprise nucleic acid constructs comprising a liver-specific promoter and a nucleic acid sequence encoding the ATP73 polypeptide, a variant of the ATP73 polypeptide or a polypeptide having at least 90% identity. The compositions can be formulated for hydrodynamic gene delivery into the liver using DNA vectors.