The invention relates to the use of matrix cells for obtaining a micro hair follicle and to the use thereof for evaluating the effect of cosmetic, pharmaceutical or dermatological products and also for the prophylactic or therapeutic treatment of a state of reduced pilosity.

Provided is a medium composition for differentiation of a human induced pluripotent stem cell to a dermal papilla precursor cell, a differentiation method, and a use for inducing hair follicle neogenesis using the differentiated dermal papilla precursor cell. Further provided is a method for differentiating a human induced pluripotent stem cell into a dermal papilla precursor cell having hair follicle forming ability and a composition of a dermal papilla precursor cell specific differentiation medium for the above differentiation, and have effectively induced hair follicle neogenesis consisting only of human cells without conventional mouse-human hybrid hair follicles by using the human induced dermal papilla precursor cell and a human induced epidermal precursor cell obtained through the differentiation method. Human hair follicle tissue produced is expected to be useful as a therapeutic method for patients suffering from hair loss by overcoming the limitations of hair loss treatments.

The problem to be solved by the present invention is to provide a serum-free medium suitable for culturing of DS cells. The present invention relates to a serum-free medium for culturing of DS cells containing platelet-derived growth factor (PDGF), or to a method for culturing of dermal sheath (DS) cells, using serum-free medium comprising PDGF.

The present disclosure relates to a serum-free medium for culturing of DS cells containing platelet derived growth factor (PDGF), or to a method for culturing of dermal sheath (DS) cells, using serum-free medium comprising PDGF.

Provided are a hair follicle germ having excellent hair growth-related properties, a method of producing a hair follicle germ, and a method of activating cells contained in a hair follicle germ. The hair follicle germ includes: epithelial cells; mesenchymal cells; and mesenchymal stem cells. The method of producing a hair follicle germ includes co-culturing epithelial cells, mesenchymal cells, and mesenchymal stem cells to form a hair follicle germ.

Provided is a hair follicle germ capable of forming a hair shaft-like structure in vitro simply and within a short period of time. A method of producing a hair follicle germ includes: inoculating epithelial cells and mesenchymal cells; maintaining the epithelial cells and the mesenchymal cells in a culture solution in which (a) laminin and entactin, and/or (b) type IV collagen is dispersed; and co-culturing the epithelial cells and the mesenchymal cells in a culture solution to form a hair follicle germ.

To provide means for efficient growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs. Provided are a culture medium for growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs, the culture medium comprising basal medium and at least (1) through (3), below: and a method for growing the epithelial cells using the culture medium: (1) at least one species of BMP inhibitor: (2) at least one species of fibroblast growth factor: and (3) at least one species of sonic hedgehog (SHH) and/or SHH agonist.

The present invention relates to a method for differentiating from human adipose-derived mesenchymal stem cells to dermal papilla cells using a differentiation induction medium composition in a cell culture plate for inducing differentiation, including gelatin, and to the medium composition. The plate comprising the gelatin of the present invention can exhibit the effect of inducing direct cross-differentiation from human adipose-derived mesenchymal stem cells to dermal papilla cells, and the effect of efficiently enabling mass cultivation ex vivo at low cost by using an economical material. In addition, the dermal papilla cells differentiated from human adipose-derived mesenchymal stem cells of the present invention can be used as a cell therapy composition for preventing or treating hair loss.

Provided is a method for isolation and expansion of dermal papilla cells, and more particularly, to a method of isolating dermal papilla cells from hair bulbs, isolated from scalp tissue, by chopping, and then expanding the isolated dermal papilla cells by passaging. The expanded dermal papilla cells may play an important role in hair growth, and thus the present invention may be used in various industrial fields, including the medical field and the cosmetic field.

Provided are compositions and methods comprising an epigenetic agent and a Wnt agonist for increasing proliferation of cochlear supporting cells or vestibular supporting cells, and related methods of treating inner ear hearing or balance disorders.