Embodiments of the invention relate to stem cells having conditional immune-evasion capabilities, methods of preparing cells and methods for using these stem cells to repair muscle tissue.

Biocompatible macroporous microcarriers, including microcarrier beads, microspheres, capsules, microsponges, hydrogels and other matrix forms, appropriate for use in a shaking flask or bioreactor to culture cells are described herein that can be used to create an edible structure for consumption or research investigation. Biocompatible, macroporous microcarriers can be dissolved or remain in the final product. Biocompatible macroporous microcarriers are formed by saccharides that are cross-linked via chemical induction with agitated cryo-gelation. Cross-linked macroporous, saccharide-microcarriers are coupled to adherence factors that enable cell binding. Finally, the cells are attached to the microcarrier for proliferation.

Disclosed herein are methods for generating satellite cells and compositions including satellite cells.

Disclosed herein are methods for generating satellite cells and compositions including satellite cells.

The invention is directed to a modified polysaccharide hydrogel, comprising a low molecular weight alginate with a specific M/G ratio. The modified polysaccharide is modified with a specific peptide, preferably comprising a cell-adhesion peptide. The modified polysaccharide hydrogel may be used as a hydrogel for the growth of cultured meat preferably as a sacrificial biopolymer.

[Problem] To provide a method for producing an artificial three-dimensional muscle tissue, said method enabling stable, efficient and easy production of a muscle tissue, and an artificial three-dimensional muscle tissue produced by this method. [Solution] A method for producing an artificial three-dimensional muscle tissue, said method comprising steps of (i) forming a three-dimensional muscle tissue precursor, which is configured from a first muscle tissue support, a second muscle tissue support and muscle cells, by linearly arranging the muscle cells on the first muscle tissue support in such a manner that the muscle cells are located close to the first muscle tissue support at one end of the line and close to the second muscle tissue support at the other end of the line, and (ii) culturing the three-dimensional muscle tissue precursor to give an artificial three-dimensional muscle tissue, and an artificial three-dimensional muscle tissue obtained by this method.

The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca.sup.2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.

The present disclosure relates to methods of preparing cell-based products for consumption that comprise proteins from exotic, endangered, and extinct species, as well as cell-based products for consumption.

The present disclosure discloses a preparation method and use of a crosslinked hydrogel for muscle stem cell culture, and belongs to the technical field of biological food materials. Chitosan, alginate, dextran and Ca.sup.2+ are crosslinked through physical crosslinking to form a double-network hydrogel with a high mechanical strength, the hydrogel is coated with heparin and collagen through dip coating, such that the hydrogel can immobilize growth factors and adhere to cells. Meanwhile, extracted primary muscle stem cells are inoculated onto the hydrogel and cultured in a growth medium (79% of DMEM, 10% of FBS and 1% of double antibodies) for 24 h. The cells are cultured in an incubator with a differential medium (97% of DMEM, 2% of horse serum and 1% of double antibodies) for 7 d. The hydrogel can enhance the absorption to nutrient substances by the muscle stem cells and facilitate growth of the muscle stem cells. The double-network hydrogel has the potential to be a scaffold for growth of muscle stem cells for cultured meat from stem cells.

The invention is in the field of cell culturing. More specifically, it is in the field of generating and expanding myogenic cells from induced pluripotent stem (iPS) cells. The invention relates inter alia to cells generated and expanded via such a method, a growth medium specifically suited for the purpose of expanding isolated myogenic cells, and methods for screening compounds on cell structures such as myotubes and myofibers.