Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the compositions and methods permit mammalian cells, tissues, and organs to be recovered from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity.

The invention relates to an additive solution for storing erythrocyte concentrates, to erythrocyte concentrates that are provided with the additive solution, and to a process for producing erythrocyte concentrates diluted with the additive solution, comprising the step of irradiation with UV light, and to the use of the additive solution for storing erythrocyte concentrates.

Described herein is a method of preserving a biological tissue sample ex vivo, the method comprising comprises contacting the tissue sample ex vivo with a perfusion solution comprising polymerized hemoglobin, wherein the perfusion solution comprises less than 5% by weight low molecular weight hemoglobin species, based on the total weight of the perfusion solution.

Provided are a cold storage method and a cold storage liquid that are suitable for non-freezing refrigerated storage of human or animal stem cells such as iPS cells or embryos in a non-frozen state. According to an embodiment, the non-freezing refrigerated storage liquid is: a mixture liquid (HTM, HTMx2c13), in which MEM-alpha (Minimum Essential Medium Eagle (MEM) Alpha Modification) is mixed with a UW solution (UW_sln; BELZER UW (registered trademark) COLD STORAGE SOLUTION), or a modified UW solution (polymer component being changed to PVA), are mixed in a ratio of about 1/1 to 1/2; or a storage liquid having a composition equivalent to the mixture liquid, especially in respect of concentrations of potassium ions and sodium ions. And, arousal period(s) of maintaining a temperature of 30 C. to 37 C. is inserted at each of two to five days, into a non-freezing refrigerated storage period, which is made at 2 C. to 8 C. for 2 to 10 days or 4 to 20 days.

A method for preparing human umbilical cord mesenchymal stem cell-derived exosomes (hUC-MSC-ES) overexpressing an ischemic myocardium-targeting peptide (IMTP) is provided, including the following steps: inserting a double-stranded fragment of SEQ ID NO: 1 into a lentiviral vector pCDH-CMV-MCS-EF1-GFP-T2A-puro to obtain a recombinant vector, co-transfecting host cells with the recombinant vector and a packaging system to obtain a lentiviral particles, infecting human umbilical cord mesenchymal stem cells (hUC-MSCs) with the lentiviral particles to obtain hUC-MSCs overexpressing the IMTP, preparing conditioned medium of the hUC-MSCs overexpressing the IMTP, and then collecting the hUC-MSC-ES overexpressing the IMTP from the conditioned medium. HUC-MSC-ES prepared by the preparation method and use thereof, as well as a pharmaceutical composition including the hUC-MSC-ES are provided. A preservation solution for the hUC-MSC-ES is also provided.

The present invention relates to a method for preserving urinary cells or cells from another body fluid, the method comprising the step of contacting a urine sample obtained from a patient with a buffer substance suitable to create and/or maintain a pH value in the range of 6 to 8, particularly approx. 7, within said urine sample, and a formaldehyde releasing compound.

A dual-vial system, kit, and method for preservation of semen samples in mail-in semen analysis kits are described. The system comprises a dual-vial kit that comprises a first vial for collecting a semen sample of a subject, a second vial having a predetermined volume of a stabilization medium, and a transfer device for transferring a predetermined portion of the semen sample from the first vial to the second vial such that the transfer device has a volume allowing transfer of 1 part of the semen sample to be diluted in 3 parts of the stabilization medium in the second vial. Accordingly, the dual-vial kit enables preservation of semen viability for an extended period of up to 72 hours and carrying out of semen analysis within up to 72 hours of storing the semen in the stabilization medium.

The present disclosure provides, among other things, methods for the treatment of neurodegenerative diseases (ND) and other mitochondrial disorders, and compositions related thereto. Described herein are in vitro (cell culture) and in vivo (animal model) experimental examples demonstrating mitochondrial organelle transplantation (MOT) for the treatment of NDs such as amyotrophic lateral sclerosis (ALS). Furthermore, as discussed herein, MOT has been performed in five human ALS patients with positive resultsmeasurable improvement of their conditions has been observed, with no adverse events.

An object of the present invention is to provide a method for preserving a platelet in a refrigerated or frozen state while retaining more platelet functions, a platelet preservation solution for use in such a method, and the like. The present invention relates to a platelet preservation solution for refrigeration or freezing, comprising an electrolyte and an anticoagulant. The present invention also relates to a method for preserving a platelet, comprising: suspending a platelet in the platelet preservation solution according to the present invention; and preserving the suspension by refrigeration or freezing at 10 C. or lower.

The disclosure relates to composition and method for cryopreservation of mitochondria, cryopreserved composition comprising mitochondria and their therapeutic use.