The storage system is intended to provide users a device, that may securely hold biological samples within a canister in a space saving storage assembly. It is further an aim of the storage system to enable easy retrieval of the biological samples through a simple canister removal mechanism. Furthermore, the system includes a compact storage assembly that comprises multiple cells and canisters stacked together having efficient structural components that are suited for a typical storage tank for biological samples. Further improvements comprise a structural support for each canister, a corrugated panel structure forming a honeycomb canister organizational matrix, and a locking mechanism.

A bag for mixing fluid volumes includes a port at a center of a first end, a second end, a first constriction, and a second constriction. The port is configured to couple to at least one fluid line for introducing and removing fluids from the bag. The second end includes a first lobe and a second lobe. The first constriction is on the first side between the first end and the second end and the second constriction is on a second side between the first end and the second end.

A method for transporting a biological sample at hypothermic temperatures. The container can be cooled using phase change material and pump fluid through the sample. The fluid can pump through the system at a rate independent of the parameters of the biological sample. A valve can control the rate of flow of the fluid into the biological sample.

A method for transporting a biological sample at hypothermic temperatures. The container can be cooled using phase change material and pump fluid through the sample. The fluid can pump through the system at a rate independent of the parameters of the biological sample. A valve can control the rate of flow of the fluid into the biological sample.

The present invention refers to a canister assembly (1) suitable for a cryopreservation container comprising: a rigid elongated handle (2) having a hook (3) in the upper part (4) suitable for supporting said rigid elongated handle (2) in an a cryopreservation container (13), a thermal bridge (5) in the upper part (4) of said elongated handle (2) and a lower portion (6); at least two cylindrical cups (7,8) having a series of apertures (12) in the bottom side, in which said at least two cylindrical cups (7,8) are located one on top of the other sharing the same longitudinal axis, and being attached to said lower part (6) of said rigid elongated handle (2); being separated by a supporting member (9) attached in a fixed manner to the upper cylindrical cup (7); characterized in that the bottom cylindrical cup (8) is attached in a fixed manner to the lower part (6) of the handle (2) by fixing means (11) and the upper cylindrical cup (7) comprises non-slip pivot joints (10) suitable for rotating the upper cylindrical cup (7) around the handle's (2) axis.

A method for transporting a biological sample at hypothermic temperatures. The container can be cooled using phase change material and pump fluid through the sample. The fluid can pump through the system at a rate independent of the parameters of the biological sample. A valve can control the rate of flow of the fluid into the biological sample.

The present invention relates to the compositions, methods, and associated devices (1) for the cryopreservation of biological specimens that enables to freeze and thaw the entire specimen by straightforwardly protecting its three-dimensional architecture. The invention comprises compositions including reagents to protect the specimen and preserve the physiological conditions. The methods comprise fewer steps excluding the need for other solutions used in conventional methods. The methods also eliminate stressful steps for biological specimens in conventional methods including harvesting, pipetting, centrifuging, and transferring. With this invention, the entire content of the specimen can be frozen, stored long-term in a single cell culture vessel with one of the associated devices (1) described here, and then thawed while still in the device (1). The biological specimens frozen by that method render high post-thawing viability. Moreover, the invention reduces cryoprotectant-related toxic events, human errors, and contamination risk.

Using adults stem cells (ASC) including hematopoietic cell (HSC), endothelial progenitor cells and mesenchymal (MSC) stem cells; mobilizing ASC cells into the vascular system using a collection system of one of: (i) an apheresis. (ii) bone marrow; or (iii) direct collection of ASC from the peripheral blood; providing the ASC with a cryopreservation conservation container of about minus 80 degrees centigrade collecting the ASC for defrosting and placed in a sterile container; reprogramming stem cells (RSC) including at least one of (i) RSC by methylation patterns of DNA such as JMJD3 and HDAC; (ii) RSC by acetylation patterns of DNA such as HDAC; (iii) epigenetic programming with a protein of interest (ncRNA); using vectors of AAV or CMV to transfer to specific genes to provide proteins to enlarge physical conditions to lost during the aging process.

The present disclosure relates to a low-temperature preservation cabinet for a biological sample, including: a cabinet, the cabinet being provided with a storage region and an equipment region. The storage region is configured to store the biological sample. The temperature isolation region is provided between the storage region and the equipment region. A temperature of the storage region is lower than a temperature of the equipment region.