Patent classifications
C12N9/2437
CLEANING COMPOSITION COMPRISING A DISPERSIN AND A CARBOHYDRASE
Cleaning compositions may include a mix of enzymes that include a dispersin and a carbohydrase. Said compositions may be used in cleaning processes and/or for deep cleaning of organic stains.
Mutant Filamentous Fungus, and Method for Producing Protein Using Same
A method for producing a mutant filamentous fungus. The method comprises modifications of XYR1 and ACE3 expression in the parent filamentous fungus. The modification of XYR1 is substitution, deletion, insertion, or addition of at least one amino acid residue in a region corresponding to positions 810 to 833 of SEQ ID NO: 1 in a polypeptide that consists of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having at least 90, identity thereto and functions as a transcriptional activator of cellulase and hemicellulase, and the modification of ACE3 expression is enhanced expression of a partial polypeptide of ACE3.
Polypeptides Having Cellobiohydrolase Activity And Polynucleotides Encoding Same
The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
COMPOSITIONS FOR ENHANCED ENZYME PRODUCTION
The present invention relates to compositions to induce production of proteins, e.g., enzymes, e.g., amylases or biomass degrading enzymes in a host cell, and methods for increasing the yield of the proteins, e.g., enzymes produced. Such compositions comprise a caramelized sugar product. The methods described herein can also be used to enhance processing of biomass materials, e.g., to produce sugar products.
Stabilized cellulase variants
Disclosed is variants of a cellulase having improved stability in the presence of a protease, and the use of such variants in laundry.
Processes for producing one or more fermentation products in a second fermentation
Processes and systems for recovering products from a fermentation mash. In some examples, a process for recovering products from a fermentation mash can include processing a ground corn product to produce a fermentation mash that can include ethanol. At least a portion of the ethanol can be separated from the fermentation mash to produce a whole stillage. The whole stillage can be separated to produce a fiber rich product and a filtrate. The fiber rich product can be hydrolyzed to produce a saccharification mash. The saccharification mash can be processed to produce additional ethanol and a stillage protein product.
Compositions for saccharification of cellulosic material
The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE
- Elena Brevnova ,
- John E. McBride ,
- Erin Wiswall ,
- Kevin S. Wenger ,
- Nicky Caiazza ,
- Heidi Hau ,
- Aaron Argyros ,
- Frank Agbogbo ,
- Charles F. Rice ,
- Trisha Barrett ,
- John S. Bardsley ,
- Abigail Foster ,
- Anne K. Warner ,
- Mark Mellon ,
- Ryan Skinner ,
- Indraneel Shikhare ,
- Riaan Den Haan ,
- Chhayal V. Gandhi ,
- Alan Belcher ,
- Vineet B. Rajgarhia ,
- Allan C. Froehlich ,
- Kristen M. Deleault ,
- Emily Stonehouse ,
- Shital A. Tripathi ,
- Jennifer Gosselin ,
- Yin-Ying Chiu ,
- Haowen Xu
The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.
Fungus Strain Having Decreased Viscosity
The invention relates to a strain of fungus having a reduced viscosity, wherein the ID 78713 (GEL3) gene has been invalidated. The invention also relates to the different uses of this strain, as well as to the method of genetic modification.
Process for producing enzymes with a strain belonging to a filamentous fungus
A process for producing cellulolytic and/or hemicellulolytic enzymes with a strain of microorganism belonging to the family of filamentous fungi. The process includes growing the fungi in an aqueous culture medium, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor. It also includes the production of enzymes, starting with the aqueous culture medium in the presence of at least one inductive carbon-based substrate and also inducing the production of hydrophobins. Further, at least a portion of the hydrophobins produced in step (b) are reintroduced into the growth step (a).