The invention relates to barley plants having a high α-amylase activity. The barley plants of the invention may for example carry a mutation in one or more α-amylase promoters, in the HRT gene, in the HBL12 gene and/or in the WRKY38 gene. The invention further provides plant products prepared from said barley plants.

Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.

The invention is directed to a method for producing conformational-stabilized proteins by incorporating a recognition sequence in a protein, the method comprising the steps of (a) generating at least one genetic construct comprising the recognition sequence; (b) expressing in a natural host the at least one genetic construct using oligonucleotide primers, thereby forming a vector; and (c) using a plant-based over-expression system with a constitutive promoter to over-express the vector. The method is remarkable in that the recognition sequence comprises at least one stretch of amino acids with as sequence Phe-x1-x2-Tyr, wherein Phe is phenylalanine, X1 and X2 are amino acid residues and Tyr is tyrosine.

Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.

Methods are disclosed for distinguishing and identifying plants by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. Methods also are disclosed for identifying the source organism of a heterologous polysaccharide-hydrolyzing enzyme in a plant by measuring partial hydrolysis of polysaccharides on account of polysaccharide-hydrolyzing enzyme activity at pre-determined incubation times and temperatures. The reaction mixture has unique chemical and physical properties that can be used to construct viscosity curves for measuring polysaccharide-hydrolyzing enzyme activity. The viscosity curves can be compared among plants to distinguish or identify the plants from one another. Likewise, viscosity curves can be compared among source organisms to identify the source organism of the heterologous polysaccharide-hydrolyzing enzyme in the plant.

The present disclosure relates generally to wheat plants comprising a genetic modification leading to reduced expression of at least one Amy 1 gene on chromosome 6B. The present disclosure further relates to grain from such plants and to products derived from the grain.

A method for producing grain with improved fuel traits is presented, whereby the fuel trait is introduced directly into the grain through pollen applied from a donor source produced separate from the farmer's field, applied to the farmers field at the proper time, thereby eliminating multiple steps of trait introgression currently in practice for such grain production.

Microfibrillated cellulose, derived from vegetables or wood, can be used to provide a liquid composition which is compatible with a broad range of ingredients suitable for consumer applications, including enzymes, while still providing good structuring of the liquid composition, without affecting ease of pour.

The invention relates to barley plants having a high -amylase activity. The barley plants of the invention may for example carry a mutation in one or more -amylase promoters, in the HIRT gene, in the HBL12 gene and/or in the WRKY38 gene. The invention further provides plant products prepared from said barley plants.