The present invention relates to “a heterodimer which combines a Factor G α-subunit containing an amino acid sequence that is the same as or substantially the same as an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4 with a Factor G β-subunit containing an amino acid sequence that is the same as or substantially the same as an amino acid sequence represented by any one of SEQ ID NO: 6, 8, 10, 12, 14, or 16, a method of measuring a β-glucan using the heterodimer, and a kit for measuring a β-glucan containing the heterodimer”.

The present application provides materials and methods for treating a patient with one or more conditions associated with PCSK9 whether ex vivo or in vivo. In addition, the present application provides materials and methods for editing and/or modulating the expression of PCSK9 gene in a cell by genome editing.

Embodiments described herein relate to a recombinant nucleic acid construct comprising a nucleic acid molecule encoding a polypeptide having at least 90% sequence identity with the amino acid sequence, or fragment thereof, of SEQ ID NO:2; SEQ ID NO:4; SEQ ID NO; 6: SEQ ID NO:8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; or SEQ ID NO: 16, as well as keratin-degrading compositions, methods of recombinantly producing a polypeptide, and methods of degrading keratin.

The present invention belongs to the field of biotechnology. More specifically, the present invention provides a protease, a non-naturally occurring fusion protein comprising a corresponding protease recognition site, expression vectors encoding same, host cells comprising said expression vectors, kit of parts as well as methods applying the protease, fusion protein, and uses thereof, as defined in the claims. The presently disclosed protease/protease recognition site is particularly useful in methods requiring an orthogonal set of proteases, and is suitable for use in both prokaryotic and selected eukaryotic expression systems

The present invention relates to a heterodimer which combines a Factor G -subunit containing an amino acid sequence that is the same as or substantially the same as an amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4 with a Factor G -subunit containing an amino acid sequence that is the same as or substantially the same as an amino acid sequence represented by any one of SEQ ID NO: 6, 8, 10, 12, 14, or 16, a method of measuring a -glucan using the heterodimer, and a kit for measuring a -glucan containing the heterodimer.

Embodiments described herein relate to a recombinant nucleic acid construct comprising a nucleic acid molecule encoding a polypeptide having at least 90% sequence identity with the amino acid sequence, or fragment thereof, of SEQ ID NO:2; SEQ ID NO:4; SEQ ID NO: 6; SEQ ID NO:8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; or SEQ ID NO: 16, as well as keratin-degrading compositions, methods of recombinantly producing a polypeptide, and methods of degrading keratin.