The present invention discloses a S-adenosyl-L-methionine δ24-sterol-C-methyltransferase mutant C24MTgm-M11. Strain MG1655 (ΔptsG ΔfumAC ΔfumB, panD, aspA, C24MTgm) is constructed based on the polynucleotide encoding the enzyme mutant. Strain MG1655 (Δpts GΔfumAC ΔfumB, panD, aspA, C24MTgm-M11) can produce 480 mg/L 3-aminoisobutyric acid under shake flask fermention. Compared to the wild type strain C24MTgm, the strain containing mutant C24MTgm-M11 has a significantly improved ability to produce 5.8 times' 3-aminobutyric acid.

A microorganism is transformed to be capable of producing guanidinoacetic acid (GAA). A method can be used for the fermentative production of GAA using such a microorganism. A corresponding method can be used for the fermentative production of creatine.

A microorganism is transformed to be capable of producing guanidinoacetic acid (GAA). A method can be used for the fermentative production of GAA using such a microorganism. A corresponding method can be used for the fermentative production of creatine.

The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

The present relates to a yeast strain and use thereof and a preparation method of ergothioneine. The present invention relates to the field of biotechnology. The yeast strain is obtained through traditional mutagenesis and screening, and its deposit number is CCTCC M 20211505. The present invention provides a preparation method of ergothioneine. The preparation method of ergothioneine comprises: mixing the aforementioned yeast strain with a fermentation medium and an optional substrate, fermenting, and then homogenizing cells and separating to obtain ergothioneine. The aforementioned yeast strain can be used for the preparation of ergothioneine, and the ergothioneine prepared by the yeast strain has the advantages of high yield, low cost and fast preparation speed. The preparation method has the advantages of low cost, environmental protection, high product quality, high yield, less impurities, less drug residues, short fermentation period and the like.

The present relates to a yeast strain and use thereof and a preparation method of ergothioneine. The present invention relates to the field of biotechnology. The yeast strain is obtained through traditional mutagenesis and screening, and its deposit number is CCTCC M 20211505. The present invention provides a preparation method of ergothioneine. The preparation method of ergothioneine comprises: mixing the aforementioned yeast strain with a fermentation medium and an optional substrate, fermenting, and then homogenizing cells and separating to obtain ergothioneine. The aforementioned yeast strain can be used for the preparation of ergothioneine, and the ergothioneine prepared by the yeast strain has the advantages of high yield, low cost and fast preparation speed. The preparation method has the advantages of low cost, environmental protection, high product quality, high yield, less impurities, less drug residues, short fermentation period and the like.

Described is a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1).

Described is a method for the incorporation of formaldehyde into biomass comprising the following enzymatically catalyzed steps (1) condensation of pyruvate with formaldehyde into 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the thus produced 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of thus produced homoserine to threonine; (4) conversion of the thus produced threonine into glycine and acetaldehyde or acetyl-CoA; (5) condensation of the thus produced glycine with formaldehyde to produce serine; and (6) conversion of the thus produced serine to produce pyruvate, wherein said pyruvate can then be used as a substrate in step (1).

The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.