A fermentation process includes contacting a starch hydrolysate with a glucoamylase with agitation. And allowing for settling to form a multi-phase solution. A first phase of the multi-phase solution includes a saccharide component comprising about 30 wt % to about 80 wt % (e.g., 30 wt % to 70 wt %, 30 wt % to 60 wt %) based on a total carbohydrate present. The method further includes draining the first phase to isolate the first phase from a second phase to form a fermentation broth comprising a first portion of the first phase. The method further includes fermenting the fermentation broth until a concentration of glucose in the fermentation broth is 40 g/L or less. The method includes adding a second portion of the first phase to the fermentation broth to maintain a concentration of glucose in a range of from about 1 g/L to about 20 g/L in the fermentation broth.

A method for producing alcoholic beverages from alkaline cooking, using as a basis the increase in pH in the medium to cook cacahuazintle corn grains, other similar mealy corn or any other grain, which offers a different proposal due to the generation of flavors, aromas and sensory characteristics that differentiate the products obtained by the methods herein disclosed from existing alcoholic beverages. A method for producing alcohol for human consumption and/or industrial use from the recovery and treatment of nejayote obtained from alkaline cooking residues, also known as nixtamalization of corn, applied to any alkaline cooking residue in the alimentary field.

Provided are ribulose-phosphate 3-epimerase, a microorganism and a composition, each including the ribulose-phosphate 3-epimerase, and a method of producing psicose-6-phosphate or psicose using the same.

Provided are ribulose-phosphate 3-epimerase, a microorganism and a composition, each including the ribulose-phosphate 3-epimerase, and a method of producing psicose-6-phosphate or psicose using the same.

A method and use of nisin-permeabilized microbial cells as whole-cell catalysts for reducing the amount of a target substrate in a sample to one of more product are provided. Specifically, a method of reducing the amount of lactose in a dairy sample using nisin-permeabilized lactic acid bacterial cell catalysts, which have been permeabilized by incubating with a nisin producing microbial cell and/or culture medium derived therof. Further provided is a nisin producing microbial cell, derived from parent strain Lactococcus lactis subsp. lactis bv. diacetylactis SD96 (NCBI accession No. SRX6686433).

The present invention relates to a method for effectively utilizing fructose raffinate obtained in the process for separating psicose conversion product with a high purity chromatography in the process for preparing psicose, and more specifically, it is utilized for preparation of fructose-containing raw material solution for preparing psicose by supplying the fructose raffinate obtained in the separation step of psicose preparation into the psicose conversion reaction.

The invention provide a system for locating an object in a volume of space, comprising: a communication device (CD) of the object disposed in the volume of space, wherein the communication device comprises a CD radio frequency (RF) receiver, a CD RF transmitter, and a CD infrared (IR) receiver; a first electrical device of a plurality of electrical devices disposed in the volume of space, wherein the first electrical device comprises at least one first sensor, a first RF receiver, a first IR transmitter, and a first RF transmitter, wherein the first RF transmitter broadcasts a first communication signal, wherein the first IR transmitter broadcasts a second communication signal, and wherein the first RF receiver receives, in response to the first communication signal received by the CD RF receiver and the second communication signal received by the CD IR receiver, a third communication signal broadcast by the CD RF transmitter of the communication device; and a controller communicably coupled to the plurality of electrical devices, wherein the controller receives a fourth communication signal sent by the first RF transmitter of the first electrical device, wherein the fourth communication signal comprises a first identification of the object and a second identification of the first electrical device, wherein the fourth communication signal is associated with a signal strength of the third communication signal received by the first RF receiver of the first electrical device.

The invention provide a system for locating an object in a volume of space, comprising: a communication device (CD) of the object disposed in the volume of space, wherein the communication device comprises a CD radio frequency (RF) receiver, a CD RF transmitter, and a CD infrared (IR) receiver; a first electrical device of a plurality of electrical devices disposed in the volume of space, wherein the first electrical device comprises at least one first sensor, a first RF receiver, a first IR transmitter, and a first RF transmitter, wherein the first RF transmitter broadcasts a first communication signal, wherein the first IR transmitter broadcasts a second communication signal, and wherein the first RF receiver receives, in response to the first communication signal received by the CD RF receiver and the second communication signal received by the CD IR receiver, a third communication signal broadcast by the CD RF transmitter of the communication device; and a controller communicably coupled to the plurality of electrical devices, wherein the controller receives a fourth communication signal sent by the first RF transmitter of the first electrical device, wherein the fourth communication signal comprises a first identification of the object and a second identification of the first electrical device, wherein the fourth communication signal is associated with a signal strength of the third communication signal received by the first RF receiver of the first electrical device.

Methods and systems are described herein for manufacturing oligosaccharides, including maltosyl-isomaltooligosaccharides. The methods involve removing undesired components from fermentation fluids that contain maltosyl-isomaltooligosaccharides.

The present invention relates to a method for converting at least one oligo- and/or polysaccharide into fructose comprising the steps of: a) adding to a composition comprising water, phosphate and at least one oligo- and/or polysaccharide at least four enzymes, and b) subsequently enzymatically converting the at least one oligo- and/or polysaccharide to fructose in the presence of the at least four enzymes, wherein in step a) at least one additional saccharide is added, whereby the at least one additional saccharide is selected from the group consisting of saccharides comprising 20 or less monosaccharide residues and/or combinations thereof; wherein in step a) the at least four enzymes, preferably at least five enzymes, are selected from the group consisting of transferases, phosphorylases, mutases, isomerases, hydrolases, phosphatases and combinations thereof; and wherein at least one enzyme in step a) is a phosphatase.