The invention provides a process of incorporation of omega-3 fatty acids such as EPA/DHA into polar lipid molecules present in lecithin, which consists of: (a) an enzymatic exchange reaction between the fatty acids present in the polar lipids of lecithin and the omega-3 fatty acids present in concentrated fish oil, to obtain an oil with a high content of polar lipids and omega-3 fatty acids and (b) a stage of concentration of the polar lipid content of the oil obtained in stage a, by supercritical fractionation or molecular distillation. The composition of the invention promotes at least a 25% peak incremental concentration in plasma of the EPA/DHA when compared to a krill oil derived composition.

The disclosure discloses a method for efficient biosynthesis of Reb D by glycosyltransferase, belonging to the field of biocatalytic synthesis. According to the disclosure, a glycosyltransferase having an activity to catalyze synthesis of Reb D from Reb A is obtained, and a mutant YojK-I241T/G327N with high catalytic activity is obtained through directed evolution. The glycosyltransferase mutant YojK-I241T/G327N and a sucrose synthase AtSuSy derived from Arabidopsis thaliana are used for constructing a coupling reaction to realize efficient catalytic synthesis of Reb D with Reb A as a substrate. The reaction is carried out by using 19.32 g/L (20 mmol/L) of Reb A as the substrate for 15 h to efficiently synthesize 20.59 g/L of Reb D, and the yield of Reb D reaches 91.29%, which provides an efficient and green new pathway for production of Reb D.

The subject invention provides microbe-based products, as well as their use to improve oil production and refining efficiency by, for example, remediating the disposable layers in oil tanks and other oil storage units. In preferred embodiments, the microbe-based products comprise biochemical-producing yeast and growth by-products thereof, such as, e.g., biosurfactants. The subject invention can be used to remediate rag layer and/or other dissolved solid layers that form in water-oil emulsions. Furthermore, the subject invention can be used for remediating solid impurities, such as sand, scale, rust and clay, in produced water, flow-back, brine, and/or fracking fluids.

The subject invention provides microbe-based products, as well as their use to improve oil production and refining efficiency by, for example, remediating the disposable layers in oil tanks and other oil storage units. In preferred embodiments, the microbe-based products comprise biochemical-producing yeast and growth by-products thereof, such as, e.g., biosurfactants. The subject invention can be used to remediate rag layer and/or other dissolved solid layers that form in water-oil emulsions. Furthermore, the subject invention can be used for remediating solid impurities, such as sand, scale, rust and clay, in produced water, flow-back, brine, and/or fracking fluids.

This disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria having a reduced or abolished synthesis of poly-N-acetyl-glucosamine (PNAG), Enterobacterial Common Antigen (ECA), cellulose, colanic acid, core oligosaccharides, Osmoregulated Periplasmic Glucans and Glucosylglycerol (O), glycan, and trebalose. The disclosure further provides methods for the production of bioproduct by the viable bacteria and uses thereof. Furthermore, the disclosure is in the technical field of fermentation of metabolically engineered microorganisms producing bioproduct.

The subject invention provides materials and methods for producing, isolating, extracting and purifying single-molecule products. The subject invention provides materials and methods for extracting microbial metabolites at a high level of purity, for example, a purity of at least 80% by weight, and preferably at least 95% by weight or more. Specifically, the subject invention provides materials and methods for isolating or extracting biosurfactants and polyketides at a high level of purity. Preferably, the biosurfactant is a sophorolipid (SLP).

The subject invention provides materials and methods for producing, isolating, extracting and purifying single-molecule products. The subject invention provides materials and methods for extracting microbial metabolites at a high level of purity, for example, a purity of at least 80% by weight, and preferably at least 95% by weight or more. Specifically, the subject invention provides materials and methods for isolating or extracting biosurfactants and polyketides at a high level of purity. Preferably, the biosurfactant is a sophorolipid (SLP).

The subject invention provides methods and compositions for replacing chemical surfactants for use in a wide variety of industrial applications. More specifically, the subject invention provides for the production of multi-functional biological surface-active compositions having one or more precise functional characteristics based on the desired use.

The subject invention provides methods and compositions for replacing chemical surfactants for use in a wide variety of industrial applications. More specifically, the subject invention provides for the production of multi-functional biological surface-active compositions having one or more precise functional characteristics based on the desired use.

The disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria. In particular, the disclosure provides viable bacteria with mutated outer membrane biosynthetic pathway leading to disruption of the pathway, preferably substantially lacking lipopolysaccharide (LPS, endotoxin) within the outer membrane. The disclosure further provides methods of generating viable bacteria and uses thereof. The disclosure also provides compositions and methods for inducing immune responses and for researching and developing therapeutic agents. Furthermore, the disclosure is in the technical field of fermentation of metabolically engineered microorganisms producing bioproduct or metabolite.