The present invention provides a method for preparing a mogroside having no β-1,6-glucoside bond comprising the step of reacting glycosidase ASBGL2, AOBGL2, AOBGL1, ASBGL1, or a variant thereof with a mogroside having at least one β-1,6-glucoside bond, thereby cleaving said β-1,6-glucoside bond.

The present invention provides a method for preparing a mogroside having no β-1,6-glucoside bond comprising the step of reacting glycosidase ASBGL2, AOBGL2, AOBGL1, ASBGL1, or a variant thereof with a mogroside having at least one β-1,6-glucoside bond, thereby cleaving said β-1,6-glucoside bond.

This disclosure describes biological pesticides that include biological mannosylerythritol lipids (MELs), and their application. Provided MEL-based pesticides are microbially produced by through microbial (fungal) fermentation of plant-based derivatives or plant-derived materials as a substrate. The biologically active components are obtained from multiple stage bio-processes including transformation, biochemical reaction, extraction, and other processing of raw materials. The synthesis, separation, concentration, purification, preparation of biological pesticides, and their application as a crop pathology treatment, are described. These biological pesticides may be used disease prevention and control for crops and other plants.

This disclosure describes biological pesticides that include biological mannosylerythritol lipids (MELs), and their application. Provided MEL-based pesticides are microbially produced by through microbial (fungal) fermentation of plant-based derivatives or plant-derived materials as a substrate. The biologically active components are obtained from multiple stage bio-processes including transformation, biochemical reaction, extraction, and other processing of raw materials. The synthesis, separation, concentration, purification, preparation of biological pesticides, and their application as a crop pathology treatment, are described. These biological pesticides may be used disease prevention and control for crops and other plants.

A process can be used for enzymatic preparation of sorbitan carboxylates. Sorbitan carboxylates are obtainable by this process.

A process for producing a solid material comprising isomaltulose crystals and trehalulose, including: A) contacting an enzyme complex with a sucrose-containing solution; B) isomerizing at least a part of the sucrose to isomaltulose and trehalulose; C) separating off the enzyme complex to obatin a solution including isomaltulose, trehalulose and water; D) partially removing the water by evaporation to obtain a concentrated solution; E) bringing the concentrated solution to a temperature range of 30° C. to 63° C. and subsequently inducing isomaltulose crystallization in this temperature range followed by cooling, thereby obtaining a solid material including isomaltulose crystals and trehalulose.

A process for producing a solid material comprising isomaltulose crystals and trehalulose, including: A) contacting an enzyme complex with a sucrose-containing solution; B) isomerizing at least a part of the sucrose to isomaltulose and trehalulose; C) separating off the enzyme complex to obatin a solution including isomaltulose, trehalulose and water; D) partially removing the water by evaporation to obtain a concentrated solution; E) bringing the concentrated solution to a temperature range of 30° C. to 63° C. and subsequently inducing isomaltulose crystallization in this temperature range followed by cooling, thereby obtaining a solid material including isomaltulose crystals and trehalulose.

Surfactants based on a newly discovered class of compounds include a hydrophobic lipid oligomer covalently linked to a peptide or peptide-like chain and a carbohydrate moiety, and a serine-leucinol dipeptide linked to the lipid oligomer. Such surfactants can be used to create an oil-in-water or water-in-oil emulsion by mixing together a polar component; a non-polar component; and the surfactant. Biosurfactants of the newly discovered class can be made by isolating and culturing a microorganism which produces the biosurfactant, and then isolating the biosurfactant from the culture. A microorganism can be engineered to produce biosurfactant of this newly discovered class by expressing a set of heterologous genes involved in the biosynthesis of the biosurfactant in the microorganism.

Surfactants based on a newly discovered class of compounds include a hydrophobic lipid oligomer covalently linked to a peptide or peptide-like chain and a carbohydrate moiety, and a serine-leucinol dipeptide linked to the lipid oligomer. Such surfactants can be used to create an oil-in-water or water-in-oil emulsion by mixing together a polar component; a non-polar component; and the surfactant. Biosurfactants of the newly discovered class can be made by isolating and culturing a microorganism which produces the biosurfactant, and then isolating the biosurfactant from the culture. A microorganism can be engineered to produce biosurfactant of this newly discovered class by expressing a set of heterologous genes involved in the biosynthesis of the biosurfactant in the microorganism.

The invention provides a process of incorporation of omega-3 fatty acids such as EPA/DHA into polar lipid molecules present in lecithin, which consists of: (a) an enzymatic exchange reaction between the fatty acids present in the polar lipids of lecithin and the omega-3 fatty acids present in concentrated fish oil, to obtain an oil with a high content of polar lipids and omega-3 fatty acids and (b) a stage of concentration of the polar lipid content of the oil obtained in stage a, by supercritical fractionation or molecular distillation. The composition of the invention promotes at least a 25% peak incremental concentration in plasma of the EPA/DHA when compared to a krill oil derived composition.