The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

A method for preparing a fermented drink is described. The method comprises the initiation of fermentation by inoculating the fermentable drink with fermentative bacteria in the form of biofilm.

A method for preparing a fermented drink is described. The method comprises the initiation of fermentation by inoculating the fermentable drink with fermentative bacteria in the form of biofilm.

The present invention relates to polypeptides which are Gal2 variants comprising at least one amino acid substitution at a position corresponding to T354, and optionally further amino acid substitution(s). The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of bioethanol and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of bioethanol and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing pentose(s), preferably D-xylose and/or L-arabinose.

The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

The present disclosure relates to methods for using one or more polypeptides with phytase activity in grain processing, ethanol, and biofuel production.

Genetically modified microorganisms useful in fermentation and methods of using such microorganisms are provided. Such microorganisms contain a [GAR+] prion or are modified to contain a [GAR+] prion. Exemplary microorganisms include yeast such as S. cerevisiae. The microorganisms can be further modified to convert xylose and/or arabinose. Methods of fermentation using such microorganisms exhibit improved fermentation efficiency and improved microorganism viability.

The present disclosure relates to using a source of one or more monosaccharides derived from a pulp or paper mill waste by-product for propagating microorganisms (e.g., yeast or bacteria). If desired, after propagation, the microorganisms can then be used to ferment one or more monosaccharides derived from a pulp or paper mill waste by-product into one or more biochemicals. Optionally, a stillage composition can be included in propagation medium to facilitate propagation and/or a stillage composition can be used to facilitate enzymatic hydrolysis of oligosaccharides and/or polysaccharides in a pulp or paper mill waste by-product to form monosaccharides.

The present disclosure relates to using a source of one or more monosaccharides derived from a pulp or paper mill waste by-product for propagating microorganisms (e.g., yeast or bacteria). If desired, after propagation, the microorganisms can then be used to ferment one or more monosaccharides derived from a pulp or paper mill waste by-product into one or more biochemicals. Optionally, a stillage composition can be included in propagation medium to facilitate propagation and/or a stillage composition can be used to facilitate enzymatic hydrolysis of oligosaccharides and/or polysaccharides in a pulp or paper mill waste by-product to form monosaccharides.