The subject invention provides materials and methods for producing and purifying sophorolipids (SLP). More specifically, the subject invention provides materials and methods for the purification of both hydrophobic and hydrophilic SLP molecules to a purity of, for example, at least 80% by weight, preferably at least 95% by weight, without using solvents or centrifugation. Advantageously, the subject invention is suitable for industrial scale production of purified SLP for use in, for example, cleaning products and detergents, and uses safe and environmentally-friendly materials and processes.

Xylitol carboxylates are products of interest in the food and cosmetics industries. A process can be used for the enzymatic preparation of xylitol carboxylates.

An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.

The invention provides a process of incorporation of omega-3 fatty acids such as EPA/DHA into polar lipid molecules present in lecithin, which consists of: (a) an enzymatic exchange reaction between the fatty acids present in the polar lipids of lecithin and the omega-3 fatty acids present in concentrated fish oil, to obtain an oil with a high content of polar lipids and omega-3 fatty acids and (b) a stage of concentration of the polar lipid content of the oil obtained in stage a, by supercritical fractionation or molecular distillation.

The present invention relates to a lipase-producing strain and application thereof. The strain is classified and named Bacillus subtilis CS1802, with a preservation number of CCTCC NO: M2018262. The strain can be used to produce vitamin A palmitate through whole-cell transformation of vitamin A and palmitic acid. The Bacillus subtilis CS1802 of the present invention is derived from traditional natural fermented food and is a microorganism generally recognized as safe. The strain can be easily cultured and preserved. The highest content of vitamin A palmitate obtained through whole-cell transformation of vitamin A and palmitic acid is 15.35 mg/L. The highest transformation efficiency is 76.75%. The strain provides a new path for enzymatic synthesis of vitamin A palmitate and has important application prospects.

The present invention relates to a lipase-producing strain and application thereof. The strain is classified and named Bacillus subtilis CS1802, with a preservation number of CCTCC NO: M2018262. The strain can be used to produce vitamin A palmitate through whole-cell transformation of vitamin A and palmitic acid. The Bacillus subtilis CS1802 of the present invention is derived from traditional natural fermented food and is a microorganism generally recognized as safe. The strain can be easily cultured and preserved. The highest content of vitamin A palmitate obtained through whole-cell transformation of vitamin A and palmitic acid is 15.35 mg/L. The highest transformation efficiency is 76.75%. The strain provides a new path for enzymatic synthesis of vitamin A palmitate and has important application prospects.

The present invention pertains to the production of a novel structurizing oil by enzymatic interesterification which novel structurizing oil is particularly well suited as oil ingredient in e.g. margarines or ice creams.

The present invention pertains to the production of a novel structurizing oil by enzymatic interesterification which novel structurizing oil is particularly well suited as oil ingredient in e.g. margarines or ice creams.

The subject invention provides improved methods for producing fatty acid esters using yeast not previously known to produce fatty acid esters. In particular, Meyerozyma spp. can be cultivated under specially-tailored conditions such that the yeast produces a variety of fatty acid esters. A yeast culture and fermentation compositions are also provided, comprising yeast cells, liquid growth medium, and a high concentration of growth by-products, such as fatty acid esters.

The subject invention provides improved methods for producing fatty acid esters using yeast not previously known to produce fatty acid esters. In particular, Meyerozyma spp. can be cultivated under specially-tailored conditions such that the yeast produces a variety of fatty acid esters. A yeast culture and fermentation compositions are also provided, comprising yeast cells, liquid growth medium, and a high concentration of growth by-products, such as fatty acid esters.