A biological toxicity test method for evaluating an ecological safety of an advanced oxidation process comprising the following steps: (1) collecting (preparing) a waste water to be determined; (2) collecting the waste water and a tap water after the advanced oxidation process treatment; (3) subjecting Koi (Cyprinus carpio haematopterus) to the water after treatment for exposure to poison; (4) Determining an anti-oxidation enzyme activity of a liver of the Koi after exposure; (5) Data analyzing. By comparing the changes of liver enzyme activities in different water, the present method evaluates the toxicity changes of micro-pollutant containing water before and after treatment, which fills in the gap of the ecological risk assessment for advanced oxidation technology.

A biological toxicity test method for evaluating an ecological safety of an advanced oxidation process comprising the following steps: (1) collecting (preparing) a waste water to be determined; (2) collecting the waste water and a tap water after the advanced oxidation process treatment; (3) subjecting Koi (Cyprinus carpio haematopterus) to the water after treatment for exposure to poison; (4) Determining an anti-oxidation enzyme activity of a liver of the Koi after exposure; (5) Data analyzing. By comparing the changes of liver enzyme activities in different water, the present method evaluates the toxicity changes of micro-pollutant containing water before and after treatment, which fills in the gap of the ecological risk assessment for advanced oxidation technology.

A kit used for fractionation of small dense LDL cholesterol (sdLDL-C) in a sample, including: a first reagent composition having one or two or more activities selected from the group consisting of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity; and a second reagent composition for quantifying the sdLDL-C, in which in an absorption spectrum after storing the first reagent composition at 37° C. for 2 weeks, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 3.00 or less, and in an absorption spectrum after storing the second reagent composition at 37° C. for 2 weeks, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 8.00 or less.

A kit used for fractionation of small dense LDL cholesterol (sdLDL-C) in a sample, including: a first reagent composition having one or two or more activities selected from the group consisting of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity; and a second reagent composition for quantifying the sdLDL-C, in which in an absorption spectrum after storing the first reagent composition at 37° C. for 2 weeks, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 3.00 or less, and in an absorption spectrum after storing the second reagent composition at 37° C. for 2 weeks, a ratio R1 represented by ABS400/ABS450 is 0.90 or more and 8.00 or less.

A personal hygiene wipe with an integral urine glucose detection feature. The wipe includes a substrate that is adapted for impregnation with a composition having one or more drying, cleaning, odor control, or antibacterial properties. The wipe also is saturated with a chemical composition for detecting glucose in urine. When glucose is detected, the wipe turns to a different color.

A personal hygiene wipe with an integral urine glucose detection feature. The wipe includes a substrate that is adapted for impregnation with a composition having one or more drying, cleaning, odor control, or antibacterial properties. The wipe also is saturated with a chemical composition for detecting glucose in urine. When glucose is detected, the wipe turns to a different color.

This invention provides a method for quantification of lipoprotein cholesterol in two steps using an autoanalyzer without pretreatment of an analyte, wherein spontaneous color development of a reagent during storage is suppressed, a kit for quantification used in the method, and a method for preparing such kit. The kit for quantification of lipoprotein cholesterol in a sample obtained from a subject used in the method for quantification of lipoprotein cholesterol in two steps comprises: (1) a first reagent composition comprising cholesterol esterase, cholesterol oxidase, cholesterol esterase, and cholesterol oxidase and leading lipoprotein cholesterol other than the analyte to the outside of the reaction system; and (2) a second reagent composition for quantifying the analyte lipoprotein cholesterol, wherein either the first reagent composition or the second reagent composition comprises at least a coupler, an iron complex, peroxidase, catalase, a hydrogen donor, and a surfactant, provided that the coupler and the hydrogen donor are not allowed to be present in the same reagent composition, and the coupler, the iron complex, and peroxidase are not allowed to be present together in either of the first reagent composition or the second reagent composition.

This invention provides a method for quantification of lipoprotein cholesterol in two steps using an autoanalyzer without pretreatment of an analyte, wherein spontaneous color development of a reagent during storage is suppressed, a kit for quantification used in the method, and a method for preparing such kit. The kit for quantification of lipoprotein cholesterol in a sample obtained from a subject used in the method for quantification of lipoprotein cholesterol in two steps comprises: (1) a first reagent composition comprising cholesterol esterase, cholesterol oxidase, cholesterol esterase, and cholesterol oxidase and leading lipoprotein cholesterol other than the analyte to the outside of the reaction system; and (2) a second reagent composition for quantifying the analyte lipoprotein cholesterol, wherein either the first reagent composition or the second reagent composition comprises at least a coupler, an iron complex, peroxidase, catalase, a hydrogen donor, and a surfactant, provided that the coupler and the hydrogen donor are not allowed to be present in the same reagent composition, and the coupler, the iron complex, and peroxidase are not allowed to be present together in either of the first reagent composition or the second reagent composition.

This invention provides a method for quantifying cholesterol in small, dense LDL in two steps using an autoanalyzer without pretreatment of an analyte, wherein spontaneous color development of a reagent during storage is suppressed, a kit for quantification used in the method, and a method for preparing such kit. The kit for quantification of cholesterol in small, dense LDL in a sample obtained from a subject used in the method for quantifying cholesterol in small, dense LDL in two steps comprises: (1) a first reagent composition having cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity and leading cholesterol in lipoproteins other than small, dense LDL to the outside of the reaction system in the presence of cholesterol esterase activity, cholesterol oxidase activity, and sphingomyelinase activity; and (2) a second reagent composition for quantifying cholesterol in small, dense LDL, wherein a coupler, an iron complex, and peroxidase activity are not allowed to be present in the same reagent composition, which is either the first reagent composition or the second reagent composition.

Sarcoidosis is a multisystem disease characterized by granulomatous inflammation in affected organs. The present invention discloses kits and a system for a blood test using mycobacterial catalase-peroxidase that has a high positive predictive value for confirming a diagnosis of sarcoidosis.