A method of identifying hydrocarbon seeps that are connected to hydrocarbon reservoirs and for identifying in situ conditions of hydrocarbon reservoirs is disclosed. The method comprises, obtaining a sample from an area of interest, such as a sediment sample or water column sample near a hydrocarbon seep; analyzing the sample to detect microbial signatures that are specific to families associated with hydrocarbon reservoirs; and using the signature to determine whether the hydrocarbon seep is connected to a hydrocarbon reservoir and to identify properties of the hydrocarbon reservoir.

Provided is a method for detecting and counting the relative content of a microorganism, comprising: adding a redox indicator to a growth medium to produce an indicating growth medium; the range of color variation of the redox indicator comprising three or more colors that can be easily recognized by the naked eye; diluting a sample to be tested, configuring multiple degrees of dilution, configuring multiple parallels for each degree of dilution, and growing the diluted test sample using the indicating growing medium; reading the color or absorbance of the indicating growth medium while growing and/or when growing is completed; and producing the relative content of a microorganism in the test sample on the basis of the level of color variation or the value of absorbance variation of the indicating growth medium.

Provided is a method for detecting and counting the relative content of a microorganism, comprising: adding a redox indicator to a growth medium to produce an indicating growth medium; the range of color variation of the redox indicator comprising three or more colors that can be easily recognized by the naked eye; diluting a sample to be tested, configuring multiple degrees of dilution, configuring multiple parallels for each degree of dilution, and growing the diluted test sample using the indicating growing medium; reading the color or absorbance of the indicating growth medium while growing and/or when growing is completed; and producing the relative content of a microorganism in the test sample on the basis of the level of color variation or the value of absorbance variation of the indicating growth medium.

A method for using genomic data to locate a reservoir is provided. The method includes collecting samples in a field over a reservoir. A genomic analysis is performed on the samples to obtain genomic data. The genomic data is clustered to classify sequences of microbial communities associated with using hydrocarbons for energy. The genomic data is used in an artificial intelligence model to identify a drilling site for hydrocarbon production.

A method for using genomic data to locate a reservoir is provided. The method includes collecting samples in a field over a reservoir. A genomic analysis is performed on the samples to obtain genomic data. The genomic data is clustered to classify sequences of microbial communities associated with using hydrocarbons for energy. The genomic data is used in an artificial intelligence model to identify a drilling site for hydrocarbon production.

The invention generally relates to analyzing yeast viability. More particularly, the invention relates to efficient and effective methods and compositions for accessing and measuring viability and concentration of yeast cells.

The invention generally relates to analyzing yeast viability. More particularly, the invention relates to efficient and effective methods and compositions for accessing and measuring viability and concentration of yeast cells.

A method for identifying in situ presence of a hydrocarbon reservoir or of a pipeline leakage is disclosed. The method can include obtaining a sample from an area of interest, such as a sediment sample or water column sample near a hydrocarbon seep or near an offshore pipeline; analyzing the sample to detect nucleic acid, protein or metabolite signatures that are indicative of cold-shock response; identifying the relative abundance of the cold-shock signatures present in the sample in comparison to the surrounding environment.

A method for identifying in situ presence of a hydrocarbon reservoir or of a pipeline leakage is disclosed. The method can include obtaining a sample from an area of interest, such as a sediment sample or water column sample near a hydrocarbon seep or near an offshore pipeline; analyzing the sample to detect nucleic acid, protein or metabolite signatures that are indicative of cold-shock response; identifying the relative abundance of the cold-shock signatures present in the sample in comparison to the surrounding environment.

Samples are collected from a first wellbore and a second wellbore. Genetic material is extracted from the samples and analyzed to determine microorganisms present in subsurface geological features through which the first wellbore and the second wellbore pass. Movement of microorganisms originating in subsurface geological features at the location of the first wellbore to subsurface geological features at the location of the second wellbore can indicate movement of a carbon-based gas between the first wellbore and the second wellbore.