Patent classifications
C12Q2304/20
Hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and <i>Salmonella </i>detection ability and a method of preparing the same
A method of preparing a hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and Salmonella detection ability includes: (1) mixing a neutral red indicator solution and distilled water at room temperature to obtain a mixture solution, and adding 1 mol/L NaHCO.sub.3 solution to the mixture solution until discoloration to obtain an alkaline solution; (2) adding an amount of the alkaline solution obtained in step (1) to a mixture of Sturgeon skin gelatin and distilled water to obtain an alkaline gelatin solution; (3) adding hydroxytyrosol and glycerin and propylene glycol to the alkaline gelatin solution to obtain a hydroxytyrosol gelatin solution, and heating the hydroxytyrosol gelatin solution at 40-70° C.; (4) ultrasonicating the hydroxytyrosol gelatin solution from step (3), placing the hydroxytyrol gelatin solution evenly on a clean plexiglass plate, drying the hydroxytyrol gelatin solution on an ultra-clean worktable to obtain the hydroxytyrosol sturgeon skin gelatin film.
HYDROXYTYROSOL STURGEON SKIN GELATIN FILM WITH ANTIOXIDANT ACTIVITY AND SALMONELLA DETECTION ABILITY AND A METHOD OF PREPARING THE SAME
A method of preparing a hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and Salmonella detection ability includes: (1) mixing a neutral red indicator solution and distilled water at room temperature to obtain a mixture solution, and adding 1 mol/L NaHCO.sub.3 solution to the mixture solution until discoloration to obtain an alkaline solution; (2) adding an amount of the alkaline solution obtained in step (1) to a mixture of Sturgeon skin gelatin and distilled water to obtain an alkaline gelatin solution; (3) adding hydroxytyrol and glycerin and propylene glycol to the alkaline gelatin solution to obtain a hydroxytyrol gelatin solution, and heating the hydroxytyrol gelatin solution at 40-70° C.; (4) ultrasonicating the hydroxytyrol gelatin solution from step (3), placing the hydroxytyrol gelatin solution evenly on a clean plexiglass plate, drying the hydroxytyrol gelatin solution on an ultra-clean worktable to obtain the hydroxytyrosol sturgeon skin gelatin film.
MICROFLUIDIC PAPER CHIP FOR DETECTING MICRO-ORGANISM, METHOD FOR PREPARING THE SAME AND METHOD FOR DETECTING MICRO-ORGANISM USING THE SAME
Disclosed is a microfluidic paper chip for detecting a microorganism, a method for preparing the same and a method for detecting the microorganism using the same. The microfluidic paper chip includes a lysis layer and a chromogenic layer which are laminated in this order. The lysis layer is composed of a hydrophilic paper containing a lysis reagent composition and the chromogenic layer is composed of a hydrophilic paper containing a chromogenic reagent.
Rapid peptidoglycan-based assay for detection of bacterial contamination
The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)--D-glucan in a sample.
RAPID PEPTIDOGLYCAN-BASED ASSAY FOR DETECTION OF BACTERIAL CONTAMINATION
The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)--D-glucan in a sample.
Rapid peptidoglycan-based assay for detection of bacterial contamination
The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)--D-glucan in a sample.